pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts

doi:10.1083/jcb.143.6.1437

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J. Cell Biol., Volume 143, Number 6, December 14, 1998 1437-1446

pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts

Fabien Cubizolles,^* Vincent Legagneux,^* René Le Guellec,^* Isabelle Chartrain,^* Rustem Uzbekov,^* Chris Ford,^§ and Katherine Le Guellec^*

^* Biologie et Génétique du Développement, CNRS UPR 41, Université de Rennes I, Campus de Beaulieu, 35042 Rennes cedex, France; Cell Cycle Group, Laboratory of Electron Microscopy, A.N. Belozersky Institute, Moscow State University, 119899, Moscow, Russia; and ^§ Department of Genetics and Development, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom

We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylatedin early embryos. This maternal mRNA encodes a protein, pEg7,whose expression is strongly increased during oocyte maturation.The tissue and cell expression pattern of pEg7 indicates thatthis protein is only readily detected in cultured cells and germcells. Immunolocalization in Xenopus cultured cells indicatesthat pEg7 concentrates onto chromosomes during mitosis. A similarlocalization of pEg7 is observed when sperm chromatin is allowedto form mitotic chromosomes in cytostatic factor-arrested eggextracts. Incubating these extracts with antibodies directed againsttwo distinct parts of pEg7 provokes a strong inhibition of thecondensation and resolution of mitotic chromosomes. Biochemicalexperiments show that pEg7 associates with Xenopus chromosome-associatedpolypeptides C and E, two components of the 13S condensin.

Key words: chromatin condensation; mitotic chromosomes; Xenopus laevis; egg extracts; chromosome-associated proteins

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