Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast

doi:10.1083/jcb.143.7.1931

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J. Cell Biol., Volume 143, Number 7, December 28, 1998 1931-1945

Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast

David W. Pruyne, Daniel H. Schott, and Anthony Bretscher

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853

The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions ofcell growth. Tropomyosin localizes specifically to actin cablesand not cortical patches. Upon shifting cells with conditionallydefective tropomyosin to restrictive temperatures, actin cablesdisappear within 1 min and both the unconventional class V myosinMyo2p and the secretory vesicle-associated Rab GTPase Sec4p depolarizerapidly. Bud growth ceases and the mother cell grows isotropically.When returned to permissive temperatures, tropomyosin-containingcables reform within 1 min in polarized arrays. Cable reassemblypermits rapid enrichment of Myo2p at the focus of nascent cablesas well as the Myo2p- dependent recruitment of Sec4p and the exocystprotein Sec8p, and the initiation of bud emergence. With the lossof actin cables, cortical patches slowly assume an isotropic distributionwithin the cell and will repolarize only after restoration ofcables. Therefore, actin cables respond to polarity cues independentlyof the overall distribution of cortical patches and are able todirectly target the Myo2p-dependent delivery of secretory vesiclesand polarization of growth.

Key words: tropomyosin; polarity; actin; MYO2; SEC4

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