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J. Cell Biol.,
Volume 144, Number 2, January 25, 1999 201-211
Institut für Biologie II/Botanik, Universität Freiburg, 79104 Freiburg, Germany
In plants, light perception by photoreceptors
leads to differential expression of an enormous number
of genes. An important step for differential gene expression is the regulation of transcription factor activities. To understand these processes in light signal transduction we analyzed the three well-known members of
the common plant regulatory factor (CPRF) family
from parsley (Petroselinum crispum). Here, we demonstrate that these CPRFs, which belong to the basic-
region leucine-zipper (bZIP) domain-containing transcription factors, are differentially distributed within
parsley cells, indicating different regulatory functions
within the regulatory networks of the plant cell. In particular, we show by cell fractionation and immunolocalization approaches that CPRF2 is transported from the
cytosol into the nucleus upon irradiation due to action
of phytochrome photoreceptors. Two NH2-terminal
domains responsible for cytoplasmic localization of
CPRF2 in the dark were characterized by deletion analysis using a set of CPRF2-green fluorescent protein (GFP) gene fusion constructs transiently expressed in
parsley protoplasts. We suggest that light-induced nuclear import of CPRF2 is an essential step in phytochrome signal transduction.
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