delta -catenin, an Adhesive Junction-associated Protein Which Promotes Cell Scattering

doi:10.1083/jcb.144.3.519

This Article

	Full Text
	PDF (Full Text)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lu, Q.
	Articles by Kosik, K. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lu, Q.
	Articles by Kosik, K. S.


Social Bookmarking

	What's this?

J. Cell Biol., Volume 144, Number 3, February 8, 1999 519-532

$delta$ -catenin, an Adhesive Junction-associated Protein Which Promotes Cell Scattering

Qun Lu,^* Mercedes Paredes,^* Miguel Medina,^* Jianhua Zhou,^* Robert Cavallo, Mark Peifer, Lisa Orecchio,^* and Kenneth S. Kosik^*

^* Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280

The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of thecadherin family linked to various catenins are the principal components. $delta$ -catenin is a mammalian brain protein in the Armadillo repeatsuperfamily with sequence similarity to the adherens junctionprotein p120^ctn. We found that $delta$ -catenin can be immunoprecipitated as a complexwith other components of the adherens junction, including cadherinand $beta$ -catenin, from transfected cells and brain. The interactionwith cadherin involves direct contact within the highly conservedjuxtamembrane region of the COOH terminus, where p120^ctn also binds. In developing mouse brain, staining with $delta$ -cateninantibodies is prominent towards the apical boundary of the neuroepithelialcells in the ventricular zone. When transfected into Madin-Darbycanine kidney (MDCK) epithelial cells $delta$ -catenin colocalized withcadherin, p120^ctn, and $beta$ -catenin. The Arm domain alone was sufficient for achievinglocalization and coimmunoprecipitation with cadherin. The ectopicexpression of $delta$ -catenin in MDCK cells altered their morphology,induced the elaboration of lamellipodia, interfered with monolayerformation, and increased scattering in response to hepatocytegrowth factor treatment. We propose that $delta$ -catenin can regulateadhesion molecules to implement the organization of large cellulararrays necessary for tissuemorphogenesis.

Key words: $delta$ -catenin; armadillo; adhesive junctions; cell motility; neural development

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Kim, H., Han, J.-R., Park, J., Oh, M., James, S. E., Chang, S., Lu, Q., Lee, K. Y., Ki, H., Song, W.-J., Kim, K. (2008). {delta}-Catenin-induced Dendritic Morphogenesis: AN ESSENTIAL ROLE OF p190RhoGEF INTERACTION THROUGH AKT1-MEDIATED PHOSPHORYLATION. J. Biol. Chem. 283: 977-987 [Abstract] [Full Text]
Silverman, J. B., Restituito, S., Lu, W., Lee-Edwards, L., Khatri, L., Ziff, E. B. (2007). Synaptic Anchorage of AMPA Receptors by Cadherins through Neural Plakophilin-Related Arm Protein AMPA Receptor-Binding Protein Complexes. J. Neurosci. 27: 8505-8516 [Abstract] [Full Text]
Gee, J M W, Shaw, V E, Hiscox, S E, McClelland, R A, Rushmere, N K, Nicholson, R I (2006). Deciphering antihormone-induced compensatory mechanisms in breast cancer and their therapeutic implications. Endocr Relat Cancer 13: S77-S88 [Abstract] [Full Text]
Kausalya, P. J., Phua, D. C.Y., Hunziker, W. (2004). Association of ARVCF with Zonula Occludens (ZO)-1 and ZO-2: Binding to PDZ-Domain Proteins and Cell-Cell Adhesion Regulate Plasma Membrane and Nuclear Localization of ARVCF. Mol. Biol. Cell 15: 5503-5515 [Abstract] [Full Text]
Rodova, M., Kelly, K. F., VanSaun, M., Daniel, J. M., Werle, M. J. (2004). Regulation of the Rapsyn Promoter by Kaiso and {delta}-Catenin. Mol. Cell. Biol. 24: 7188-7196 [Abstract] [Full Text]
Vincent, P. A., Xiao, K., Buckley, K. M., Kowalczyk, A. P. (2004). VE-cadherin: adhesion at arm's length. Am. J. Physiol. Cell Physiol. 286: C987-C997 [Abstract] [Full Text]
Franz, C. M., Ridley, A. J. (2004). p120 Catenin Associates with Microtubules: INVERSE RELATIONSHIP BETWEEN MICROTUBULE BINDING AND RHO GTPase REGULATION. J. Biol. Chem. 279: 6588-6594 [Abstract] [Full Text]
Ozawa, M. (2003). p120-independent Modulation of E-cadherin Adhesion Activity by the Membrane-proximal Region of the Cytoplasmic Domain. J. Biol. Chem. 278: 46014-46020 [Abstract] [Full Text]
Davis, M. A., Ireton, R. C., Reynolds, A. B. (2003). A core function for p120-catenin in cadherin turnover. J. Cell Biol. 163: 525-534 [Abstract] [Full Text]
Martinez, M. C., Ochiishi, T., Majewski, M., Kosik, K. S. (2003). Dual regulation of neuronal morphogenesis by a {delta}-catenin-cortactin complex and Rho. J. Cell Biol. 162: 99-111 [Abstract] [Full Text]
Huang, R., Xing, Z., Luan, Z., Wu, T., Wu, X., Hu, G. (2003). A Specific Splicing Variant of SVH, a Novel Human Armadillo Repeat Protein, Is Up-Regulated in Hepatocellular Carcinomas. Cancer Res. 63: 3775-3782 [Abstract] [Full Text]
Hatzfeld, M., Green, K. J., Sauter, H. (2003). Targeting of p0071 to desmosomes and adherens junctions is mediated by different protein domains. J. Cell Sci. 116: 1219-1233 [Abstract] [Full Text]
Myster, S. H., Cavallo, R., Anderson, C. T., Fox, D. T., Peifer, M. (2003). Drosophila p120catenin plays a supporting role in cell adhesion but is not an essential adherens junction component. J. Cell Biol. 160: 433-449 [Abstract] [Full Text]
Ireton, R. C., Davis, M. A., van Hengel, J., Mariner, D. J., Barnes, K., Thoreson, M. A., Anastasiadis, P. Z., Matrisian, L., Bundy, L. M., Sealy, L., Gilbert, B., van Roy, F., Reynolds, A. B. (2002). A novel role for p120 catenin in E-cadherin function. J. Cell Biol. 159: 465-476 [Abstract] [Full Text]
Laura, R. P., Witt, A. S., Held, H. A., Gerstner, R., Deshayes, K., Koehler, M. F. T., Kosik, K. S., Sidhu, S. S., Lasky, L. A. (2002). The Erbin PDZ Domain Binds with High Affinity and Specificity to the Carboxyl Termini of delta -Catenin and ARVCF. J. Biol. Chem. 277: 12906-12914 [Abstract] [Full Text]
Izawa, I., Nishizawa, M., Ohtakara, K., Inagaki, M. (2002). Densin-180 Interacts with delta -Catenin/Neural Plakophilin-related Armadillo Repeat Protein at Synapses. J. Biol. Chem. 277: 5345-5350 [Abstract] [Full Text]
Nakagawa, M, Fukata, M, Yamaga, M, Itoh, N, Kaibuchi, K (2001). Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites. J. Cell Sci. 114: 1829-1838 [Abstract]
Troxell, M., Loftus, D., Nelson, W., Marrs, J. (2001). Mutant cadherin affects epithelial morphogenesis and invasion, but not transformation. J. Cell Sci. 114: 1237-1246 [Abstract]
Grosheva, I, Shtutman, M, Elbaum, M, Bershadsky, A. (2001). p120 catenin affects cell motility via modulation of activity of Rho-family GTPases: a link between cell-cell contact formation and regulation of cell locomotion. J. Cell Sci. 114: 695-707 [Abstract]
Kim, K., Pang, K. M., Evans, M., Hay, E. D. (2000). Overexpression of beta -Catenin Induces Apoptosis Independent of Its Transactivation Function with LEF-1 or the Involvement of Major G1 Cell Cycle Regulators. Mol. Biol. Cell 11: 3509-3523 [Abstract] [Full Text]
Noren, N. K., Liu, B. P., Burridge, K., Kreft, B. (2000). p120 Catenin Regulates the Actin Cytoskeleton via Rho Family GTPases. J. Cell Biol. 150: 567-580 [Abstract] [Full Text]
Hatzfeld, M., Haffner, C., Schulze, K., Vinzens, U. (2000). The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149: 209-222 [Abstract] [Full Text]
Gumbiner, B. M. (2000). Regulation of Cadherin Adhesive Activity. J. Cell Biol. 148: 399-404 [Abstract] [Full Text]
Kaufmann, U, Zuppinger, C, Waibler, Z, Rudiger, M, Urbich, C, Martin, B, Jockusch, B., Eppenberger, H, Starzinski-Powitz, A (2000). The armadillo repeat region targets ARVCF to cadherin-based cellular junctions. J. Cell Sci. 113: 4121-4135 [Abstract]
Thoreson, M. A., Anastasiadis, P. Z., Daniel, J. M., Ireton, R. C., Wheelock, M. J., Johnson, K. R., Hummingbird, D. K., Reynolds, A. B. (2000). Selective Uncoupling of p120ctn from E-cadherin Disrupts Strong Adhesion. J. Cell Biol. 148: 189-202 [Abstract] [Full Text]
Anastasiadis, P., Reynolds, A. (2000). The p120 catenin family: complex roles in adhesion, signaling and cancer. J. Cell Sci. 113: 1319-1334 [Abstract]
Mariner, D., Wang, J, Reynolds, A. (2000). ARVCF localizes to the nucleus and adherens junction and is mutually exclusive with p120(ctn) in E-cadherin complexes. J. Cell Sci. 113: 1481-1490 [Abstract]
Aono, S., Nakagawa, S., Reynolds, A. B., Takeichi, M. (1999). p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells. J. Cell Biol. 145: 551-562 [Abstract] [Full Text]
Fukata, M, Nakagawa, M, Kuroda, S, Kaibuchi, K (1999). Cell adhesion and Rho small GTPases. J. Cell Sci. 112: 4491-4500 [Abstract]
Paulson, A. F., Mooney, E., Fang, X., Ji, H., McCrea, P. D. (2000). Xarvcf, Xenopus Member of the p120 Catenin Subfamily Associating with Cadherin Juxtamembrane Region. J. Biol. Chem. 275: 30124-30131 [Abstract] [Full Text]
Deguchi, M., Iizuka, T., Hata, Y., Nishimura, W., Hirao, K., Yao, I., Kawabe, H., Takai, Y. (2000). PAPIN. A NOVEL MULTIPLE PSD-95/Dlg-A/ZO-1 PROTEIN INTERACTING WITH NEURAL PLAKOPHILIN-RELATED Armadillo REPEAT PROTEIN/delta -CATENIN AND p0071. J. Biol. Chem. 275: 29875-29880 [Abstract] [Full Text]
Jaulin-Bastard, F., Arsanto, J.-P., Le Bivic, A., Navarro, C., Vely, F., Saito, H., Marchetto, S., Hatzfeld, M., Santoni, M.-J., Birnbaum, D., Borg, J.-P. (2002). Interaction between Erbin and a Catenin-related Protein in Epithelial Cells. J. Biol. Chem. 277: 2869-2875 [Abstract] [Full Text]