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J. Cell Biol.,
Volume 144, Number 4, February 22, 1999 711-720
Department of Biology, University of California, San Diego, La Jolla, California 92093-0347
The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae.
Defects in mitochondrial transmission to daughter buds
and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37°C, while loss
of the mitochondrial genome occurred after 4-24 h at
the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase
previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of
the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a
conserved residue in the putative GTP-binding site was
unable to complement any of the mutant defects. It also
caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight
complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that
functions on the mitochondrial surface.
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