J. Cell Biol., Volume 144, Number 5, March 8, 1999 1019-1031

Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts

Alexandre Angers-Loustau,^* Jean-François Côté,^* Alain Charest, Donald Dowbenko,^§ Susan Spencer,^§ Laurence A. Lasky,^§ and Michel L. Tremblay^*

^* Department of Biochemistry, McGill University, Montréal, Québec, Canada H3G 1Y6;  Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; and ^§ Department of Molecular Oncology, Genentech, Inc., South San Francisco, California 94080

In this article, we show that, in transfected COS-1 cells, protein tyrosine phosphatase (PTP)-PEST translocates to the membrane periphery following stimulation by the extracellular matrix protein fibronectin. When plated on fibronectin, PTP-PEST (/) fibroblasts display a strong defect in motility. 3 h after plating on fibronectin, the number and size of vinculin containing focal adhesions were greatly increased in the homozygous PTP-PEST mutant cells as compared with heterozygous cells. This phenomenon appears to be due in part to a constitutive increase in tyrosine phosphorylation of p130^CAS, a known PTP-PEST substrate, paxillin, which associates with PTP-PEST in vitro, and focal adhesion kinase (FAK). Another effect of this constitutive hyperphosphorylation, consistent with the focal adhesion regulation defect, is that (/) cells spread faster than the control cell line when plated on fibronectin. In the PTP-PEST (/) cells, an increase in affinity for the SH2 domains of Src and Crk towards p130^CAS was also observed. In (/) cells, we found a significant increase in the level of tyrosine phosphorylation of PSTPIP, a cleavage furrow-associated protein that interacts physically with all PEST family members. An effect of PSTPIP hyperphosphorylation appears to be that some cells remain attached at the site of the cleavage furrow for an extended period of time. In conclusion, our data suggest PTP-PEST plays a dual role in cell cytoskeleton organization, by promoting the turnover of focal adhesions required for cell migration, and by directly or indirectly regulating the proline, serine, threonine phosphatase interacting protein (PSTPIP) tyrosine phosphorylation level which may be involved in regulating cleavage furrow formation or disassembly during normal cell division.

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• Nishiya, N., Tachibana, K., Shibanuma, M., Mashimo, J.-i., Nose, K. (2001). Hic-5-Reduced Cell Spreading on Fibronectin: Competitive Effects between Paxillin and Hic-5 through Interaction with Focal Adhesion Kinase. Mol. Cell. Biol. 21: 5332-5345 [Abstract] [Full Text]  
• Lu, Z., Jiang, G., Blume-Jensen, P., Hunter, T. (2001). Epidermal Growth Factor-Induced Tumor Cell Invasion and Metastasis Initiated by Dephosphorylation and Downregulation of Focal Adhesion Kinase. Mol. Cell. Biol. 21: 4016-4031 [Abstract] [Full Text]  
• WILSON, P. D. (2001). Polycystin: New Aspects of Structure, Function, and Regulation. J. Am. Soc. Nephrol. 12: 834-845 [Abstract] [Full Text]  
• Goligorsky, M. S. (2001). The concept of cellular "fight-or-flight" reaction to stress. Am. J. Physiol. Renal Physiol. 280: F551-F561 [Abstract] [Full Text]  
• Pixley, F. J., Lee, P. S. W., Condeelis, J. S., Stanley, E. R. (2001). Protein Tyrosine Phosphatase {phi} Regulates Paxillin Tyrosine Phosphorylation and Mediates Colony-Stimulating Factor 1-Induced Morphological Changes in Macrophages. Mol. Cell. Biol. 21: 1795-1809 [Abstract] [Full Text]  
• Yamada, K. M., Araki, M. (2001). Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis. J. Cell Sci. 114: 2375-2382 [Abstract] [Full Text]  
• Kruger, J., Butler, J. R., Cherapanov, V., Dong, Q., Ginzberg, H., Govindarajan, A., Grinstein, S., Siminovitch, K. A., Downey, G. P. (2000). Deficiency of Src Homology 2-Containing Phosphatase 1 Results in Abnormalities in Murine Neutrophil Function: Studies in Motheaten Mice. J. Immunol. 165: 5847-5859 [Abstract] [Full Text]  
• Yang, L., Guerrero, J., Hong, H., DeFranco, D. B., Stallcup, M. R. (2000). Interaction of the tau 2 Transcriptional Activation Domain of Glucocorticoid Receptor with a Novel Steroid Receptor Coactivator, Hic-5, Which Localizes to Both Focal Adhesions and the Nuclear Matrix. Mol. Biol. Cell 11: 2007-2018 [Abstract] [Full Text]  
• Petit, V., Boyer, B., Lentz, D., Turner, C. E., Thiery, J. P., Valles, A. M. (2000). Phosphorylation of Tyrosine Residues 31 and 118 on Paxillin Regulates Cell Migration through an Association with CRK in NBT-II Cells. J. Cell Biol. 148: 957-970 [Abstract] [Full Text]  
• Shen, Y., Lyons, P., Cooley, M., Davidson, D., Veillette, A., Salgia, R., Griffin, J. D., Schaller, M. D. (2000). The Noncatalytic Domain of Protein-tyrosine Phosphatase-PEST Targets Paxillin for Dephosphorylation in Vivo. J. Biol. Chem. 275: 1405-1413 [Abstract] [Full Text]  
• Petrone, A, Sap, J (2000). Emerging issues in receptor protein tyrosine phosphatase function: lifting fog or simply shifting?. J. Cell Sci. 113: 2345-2354 [Abstract]  
• Maheshwari, G, Brown, G, Lauffenburger, D., Wells, A, Griffith, L. (2000). Cell adhesion and motility depend on nanoscale RGD clustering. J. Cell Sci. 113: 1677-1686 [Abstract]  
• Thomas, J. W., Cooley, M. A., Broome, J. M., Salgia, R., Griffin, J. D., Lombardo, C. R., Schaller, M. D. (1999). The Role of Focal Adhesion Kinase Binding in the Regulation of Tyrosine Phosphorylation of Paxillin. J. Biol. Chem. 274: 36684-36692 [Abstract] [Full Text]  
• Cote, J.-F., Turner, C. E., Tremblay, M. L. (1999). Intact LIM 3 and LIM 4 Domains of Paxillin Are Required for the Association to a Novel Polyproline Region (Pro 2) of Protein-Tyrosine Phosphatase-PEST. J. Biol. Chem. 274: 20550-20560 [Abstract] [Full Text]  
• Sieg, D., Hauck, C., Schlaepfer, D. (1999). Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. J. Cell Sci. 112: 2677-2691 [Abstract]  
• Escalante, M., Courtney, J., Chin, W. G., Teng, K. K., Kim, J.-I., Fajardo, J. E., Mayer, B. J., Hempstead, B. L., Birge, R. B. (2000). Phosphorylation of c-Crk II on the Negative Regulatory Tyr222 Mediates Nerve Growth Factor-induced Cell Spreading and Morphogenesis. J. Biol. Chem. 275: 24787-24797 [Abstract] [Full Text]  
• Buist, A., Blanchetot, C., Tertoolen, L. G. J., den Hertog, J. (2000). Identification of p130cas as an in Vivo Substrate of Receptor Protein-tyrosine Phosphatase alpha. J. Biol. Chem. 275: 20754-20761 [Abstract] [Full Text]  
• Noguchi, T., Tsuda, M., Takeda, H., Takada, T., Inagaki, K., Yamao, T., Fukunaga, K., Matozaki, T., Kasuga, M. (2001). Inhibition of Cell Growth and Spreading by Stomach Cancer-associated Protein-tyrosine Phosphatase-1 (SAP-1) through Dephosphorylation of p130cas. J. Biol. Chem. 276: 15216-15224 [Abstract] [Full Text]  
• Cheng, A., Bal, G. S., Kennedy, B. P., Tremblay, M. L. (2001). Attenuation of Adhesion-dependent Signaling and Cell Spreading in Transformed Fibroblasts Lacking Protein Tyrosine Phosphatase-1B. J. Biol. Chem. 276: 25848-25855 [Abstract] [Full Text]  
• Lyons, P. D., Dunty, J. M., Schaefer, E. M., Schaller, M. D. (2001). Inhibition of the Catalytic Activity of Cell Adhesion Kinase beta by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation. J. Biol. Chem. 276: 24422-24431 [Abstract] [Full Text]  
• Xu, F., Zhao, R., Peng, Y., Guerrah, A., Zhao, Z. J. (2001). Association of Tyrosine Phosphatase SHP-2 with F-actin at Low Cell Densities. J. Biol. Chem. 276: 29479-29484 [Abstract] [Full Text]  
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• Sorenson, C. M., Sheibani, N. (2002). Altered regulation of SHP-2 and PTP 1B tyrosine phosphatases in cystic kidneys from bcl-2 -/- mice. Am. J. Physiol. Renal Physiol. 282: F442-F450 [Abstract] [Full Text]  

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