The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells

doi:10.1083/jcb.145.1.141

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J. Cell Biol., Volume 145, Number 1, April 5, 1999 141-151

The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells

Rosa Puertollano,^* Fernando Martín-Belmonte,^* Jaime Millán,^* María del Carmen de Marco,^* Juan P. Albar, Leonor Kremer, and Miguel A. Alonso^*

^* Centro de Biología Molecular "Severo Ochoa," Universidad Autónoma de Madrid and Consejo Superior de Investigaciones Científicas, and Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Cantoblanco, 28049-Madrid, Spain

The MAL (MAL/VIP17) proteolipid is a nonglycosylated integral membrane protein expressed in a restricted pattern of cell types,including T lymphocytes, myelin-forming cells, and polarized epithelialcells. Transport of the influenza virus hemagglutinin (HA) tothe apical surface of epithelial Madin-Darby canine kidney (MDCK)cells appears to be mediated by a pathway involving glycolipid-and cholesterol- enriched membranes (GEMs). In MDCK cells, MALhas been proposed previously as being an element of the proteinmachinery for the GEM-dependent apical transport pathway. Usingan antisense oligonucleotide-based strategy and a newly generatedmonoclonal antibody to canine MAL, herein we have approached theeffect of MAL depletion on HA transport in MDCK cells. We havefound that MAL depletion diminishes the presence of HA in GEMs,reduces the rate of HA transport to the cell surface, inhibitsthe delivery of HA to the apical surface, and produces partialmissorting of HA to the basolateral membrane. These effects werecorrected by ectopic expression of MAL in MDCK cells whose endogenousMAL protein was depleted. Our results indicate that MAL is necessaryfor both normal apical transport and accurate sorting ofHA.

Key words: apical transport; glycolipid-enriched membranes; sorting; Madin-Darby canine kidney cells; proteolipids

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