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J. Cell Biol.,
Volume 145, Number 4, May 17, 1999 689-698
* Unité de Pathogénie Microbienne Moléculaire, U389, Institut National de la Santé et de la Recherche Médicale, Institut
Pasteur, 75724 Paris Cédex 15, France; Bacterial lipopolysaccharide (LPS) at the
apical surface of polarized intestinal epithelial cells was
previously shown to be transported from the apical to
the basolateral pole of the epithelium (Beatty, W.L., and
P.J. Sansonetti. 1997. Infect. Immun. 65:4395-4404). The
present study was designed to elucidate the transcytotic
pathway of LPS and to characterize the endocytic compartments involved in this process. Confocal and electron microscopic analyses revealed that LPS internalized at the apical surface became rapidly distributed
within endosomal compartments accessible to basolaterally internalized transferrin. This compartment
largely excluded fluid-phase markers added at either
pole. Access to the basolateral side of the epithelium
subsequent to trafficking to basolateral endosomes occurred via exocytosis into the paracellular space beneath the intercellular tight junctions. LPS appeared to
exploit other endocytic routes with much of the internalized LPS recycled to the original apical membrane.
In addition, analysis of LPS in association with markers
of the endocytic network revealed that some LPS was
sent to late endosomal and lysosomal compartments.
Centre d'Immunologie-Marseille-Luminy, Parc Scientifique et Technologique de
Luminy, Case 906, 13288 Marseille Cédex 9, France; and § Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris Cédex 15, France
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