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J. Cell Biol.,
Volume 145, Number 5, May 31, 1999 993-1007
*
* Department of Pediatric Oncology, Dana-Farber Cancer Institute, § Department of Pediatric Hematology, The Children's
Hospital, and Microtubule dynamics vary during the cell
cycle, and microtubules appear to be more dynamic in
vivo than in vitro. Proteins that promote dynamic instability are therefore central to microtubule behavior in
living cells. Here, we report that a yeast protein of the
highly conserved EB1 family, Bim1p, promotes cytoplasmic microtubule dynamics specifically during G1.
During G1, microtubules in cells lacking BIM1 showed
reduced dynamicity due to a slower shrinkage rate,
fewer rescues and catastrophes, and more time spent in
an attenuated/paused state. Human EB1 was identified as an interacting partner for the adenomatous polyposis
coli (APC) tumor suppressor protein. Like human
EB1, Bim1p localizes to dots at the distal ends of cytoplasmic microtubules. This localization, together with
data from electron microscopy and a synthetic interaction with the gene encoding the kinesin Kar3p, suggests
that Bim1p acts at the microtubule plus end. Our in
vivo data provide evidence of a cell cycle-specific microtubule-binding protein that promotes microtubule dynamicity.
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115; and Boulder Laboratory for
3D Fine Structure, Boulder, Colorado 80309
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