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Epitomics: The Rabbit Monoclonal Company
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J. Cell Biol., Volume 146, Number 2, July 26, 1999 453-464
Copyright © 1999 by The Rockefeller University Press.

DAip1, a Dictyostelium Homologue of the Yeast Actin-interacting Protein 1, Is Involved in Endocytosis, Cytokinesis, and Motility

Angelika Konzoka, Igor Webera, Evelyn Simmetha, Ulrike Hackera, Markus Maniakb, and Annette Müller-Taubenbergera
a Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany
b MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, United Kingdom

Correspondence to: Annette Müller-Taubenberger, Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany., amueller{at}biochem.mpg.de (E-mail), 49-89-8578-2322/2339 (phone), 49-89-8578-3885 (fax)

The 64-kD protein DAip1 from Dictyostelium contains nine WD40-repeats and is homologous to the actin-interacting protein 1, Aip1p, from Saccharomyces cerevisiae, and to related proteins from Caenorhabditis, Physarum, and higher eukaryotes.

We show that DAip1 is localized to dynamic regions of the cell cortex that are enriched in filamentous actin: phagocytic cups, macropinosomes, lamellipodia, and other pseudopodia. In cells expressing green fluorescent protein (GFP)-tagged DAip1, the protein rapidly redistributes into newly formed cortical protrusions.

Functions of DAip1 in vivo were assessed using null mutants generated by gene replacement, and by overexpressing DAip1. DAip1-null cells are impaired in growth and their rates of fluid-phase uptake, phagocytosis, and movement are reduced in comparison to wild-type rates. Cytokinesis is prolonged in DAip1-null cells and they tend to become multinucleate. On the basis of similar results obtained by DAip1 overexpression and effects of latrunculin-A treatment, we propose a function for DAip1 in the control of actin depolymerization in vivo, probably through interaction with cofilin. Our data suggest that DAip1 plays an important regulatory role in the rapid remodeling of the cortical actin meshwork.

Key Words: actin, Dictyostelium discoideum, endocytosis, motility, WD-repeat


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