JCB logo
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Legrand, C.
Right arrow Articles by Tournier, J.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Legrand, C.
Right arrow Articles by Tournier, J.-M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
J. Cell Biol., Volume 146, Number 2, July 26, 1999 517-529
Copyright © 1999 by The Rockefeller University Press.

Airway Epithelial Cell Migration Dynamics: MMP-9 Role in Cell–Extracellular Matrix Remodeling

Claire Legranda, Christine Gillesc, Jean-Marie Zahma, Myriam Polettea,b, Anne-Cécile Buissona, Hervé Kaplana, Philippe Birembauta,b, and Jean-Marie Tourniera
a INSERM Unité 514, IFR 53, Université de Reims,
b Laboratoire Pol Bouin, Unité de Biologie Cellulaire, Centre Hospitalier Universitaire Maison Blanche, 51092 Reims, France
c Laboratory of Tumor and Developmental Biology, University of Liège, C.H.U. Sart-Tilman, B23, 4000 Liège, Belgium

Correspondence to: Jean-Marie Tournier, INSERM Unité 514, Centre Hospitalier Universitaire Maison Blanche, 45 rue Cognacq-Jay, 51092 Reims Cedex, France., jm.tournier{at}univ-reims.fr (E-mail), 33-3-26-78-77-70 (phone), 33-3-26-06-58-61 (fax)

Cell spreading and migration associated with the expression of the 92-kD gelatinase (matrix metalloproteinase 9 or MMP-9) are important mechanisms involved in the repair of the respiratory epithelium. We investigated the location of MMP-9 and its potential role in migrating human bronchial epithelial cells (HBEC). In vivo and in vitro, MMP-9 accumulated in migrating HBEC located at the leading edge of a wound and MMP-9 expression paralleled cell migration speed. MMP-9 accumulated through an actin-dependent pathway in the advancing lamellipodia of migrating cells and was subsequently found active in the extracellular matrix (ECM). Lamellipodia became anchored through primordial contacts established with type IV collagen. MMP-9 became amassed behind collagen IV where there were fewer cell–ECM contacts. Both collagen IV and MMP-9 were involved in cell migration because when cell–collagen IV interaction was blocked, cells spread slightly but did not migrate; and when MMP-9 activation was prevented, cells remained fixed on primordial contacts and did not advance at all. These observations suggest that MMP-9 controls the migration of repairing HBEC by remodeling the provisional ECM implicated in primordial contacts.

Key Words: cell migration, gelatinase, matrix metalloproteinase, bronchial epithelium, wound repair


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents