JCB logo
R&D Systems
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ferrary, E.
Right arrow Articles by Robine, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ferrary, E.
Right arrow Articles by Robine, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
J. Cell Biol., Volume 146, Number 4, August 23, 1999 819-830
Copyright © 1999 by The Rockefeller University Press.

In Vivo, Villin Is Required for Ca2+-dependent F-actin Disruption in Intestinal Brush Borders

Evelyne Ferrarya, Michel Cohen-Tannoudjib, Gérard Pehau-Arnaudeta, Alexandre Lapillonnea, Rafika Athmana, Tereza Ruiza, Lilia Boulouhaa,c, Fatima El Marjoua, Anne Doyea, Jean-Jacques Fontainec, Claude Antonya, Charles Babinetb, Daniel Louvarda, Frédéric Jaissera, and Sylvie Robinea
a Institut Curie, UMR 144, 75248 Paris, France
b Unité de Biologie du Développement, Centre National de la Recherche Scientifique, URA 1960, Institut Pasteur, 75015 Paris, France
c Ecole Nationale Vétérinaire d'Alfort, 94700 Maisons-Alfort, France

Correspondence to: Sylvie Robine, UMR 144, Laboratoire de Morphogénèse et Signalisation Cellulaires, Institut Curie, 26, rue d'Ulm, 75248 Paris cedex 05, France. Tel:33-1-42-34-63-62 Fax:33-1-42-34-63-77 E-mail:Sylvie.Robine{at}curie.fr.

Villin is an actin-binding protein localized in intestinal and kidney brush borders. In vitro, villin has been demonstrated to bundle and sever F-actin in a Ca2+-dependent manner. We generated knockout mice to study the role of villin in vivo. In villin-null mice, no noticeable changes were observed in the ultrastructure of the microvilli or in the localization and expression of the actin-binding and membrane proteins of the intestine. Interestingly, the response to elevated intracellular Ca2+ differed significantly between mutant and normal mice. In wild-type animals, isolated brush borders were disrupted by the addition of Ca2+, whereas Ca2+ had no effect in villin-null isolates. Moreover, increase in intracellular Ca2+ by serosal carbachol or mucosal Ca2+ ionophore A23187 application abolished the F-actin labeling only in the brush border of wild-type animals. This F-actin disruption was also observed in physiological fasting/refeeding experiments. Oral administration of dextran sulfate sodium, an agent that causes colonic epithelial injury, induced large mucosal lesions resulting in a higher death probability in mice lacking villin, 36 ± 9.6%, compared with wild-type mice, 70 ± 8.8%, at day 13. These results suggest that in vivo, villin is not necessary for the bundling of F-actin microfilaments, whereas it is necessary for the reorganization elicited by various signals. We postulate that this property might be involved in cellular plasticity related to cell injury.

Key Words: villin knockout, intestine, actin-binding proteins, microvilli, mouse


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents