Characterization and Dynamics of Aggresome Formation by a Cytosolic GFP-Chimera

Correspondence to: Elizabeth S. Sztul, Department of Cell Biology, MCLM, Room 668, 1918 University Boulevard, Birmingham, AL 35294. Tel:(205) 934-1465 Fax:(205) 975-9131 E-mail:esztul{at}uab.edu.

Formation of a novel structure, the aggresome, has been proposed to represent a general cellular response to the presence of misfolded proteins (Johnston, J.A., C.L. Ward, and R.R. Kopito. 1998. J. Cell Biol. 143:1883–1898; Wigley, W.C., R.P. Fabunmi, M.G. Lee, C.R. Marino, S. Muallem, G.N. DeMartino, and P.J. Thomas. 1999. J. Cell Biol. 145:481–490). To test the generality of this finding and characterize aspects of aggresome composition and its formation, we investigated the effects of overexpressing a cytosolic protein chimera (GFP-250) in cells. Overexpression of GFP-250 caused formation of aggresomes and was paralleled by the redistribution of the intermediate filament protein vimentin as well as by the recruitment of the proteasome, and the Hsp70 and the chaperonin systems of chaperones. Interestingly, GFP-250 within the aggresome appeared not to be ubiquitinated. In vivo time-lapse analysis of aggresome dynamics showed that small aggregates form within the periphery of the cell and travel on microtubules to the MTOC region where they remain as distinct but closely apposed particulate structures. Overexpression of p50/dynamitin, which causes the dissociation of the dynactin complex, significantly inhibited the formation of aggresomes, suggesting that the minus-end–directed motor activities of cytoplasmic dynein are required for aggresome formation. Perinuclear aggresomes interfered with correct Golgi localization and disrupted the normal astral distribution of microtubules. However, ER-to-Golgi protein transport occurred normally in aggresome containing cells. Our results suggest that aggresomes can be formed by soluble, nonubiquitinated proteins as well as by integral transmembrane ubiquitinated ones, supporting the hypothesis that aggresome formation might be a general cellular response to the presence of misfolded proteins.

Key Words: aggresome, p50/dynamitin, chaperones, proteasome, microtubules

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• Kolodziejska, K. E., Burns, A. R., Moore, R. H., Stenoien, D. L., Eissa, N. T. (2005). Regulation of inducible nitric oxide synthase by aggresome formation. Proc. Natl. Acad. Sci. USA 102: 4854-4859 [Abstract] [Full Text]  
• Burnett, B. G., Pittman, R. N. (2005). The polyglutamine neurodegenerative protein ataxin 3 regulates aggresome formation. Proc. Natl. Acad. Sci. USA 102: 4330-4335 [Abstract] [Full Text]  
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• Harada, M., Kawaguchi, T., Kumemura, H., Terada, K., Ninomiya, H., Taniguchi, E., Hanada, S., Baba, S., Maeyama, M., Koga, H., Ueno, T., Furuta, K., Suganuma, T., Sugiyama, T., Sata, M. (2005). The Wilson Disease Protein ATP7B Resides in the Late Endosomes with Rab7 and the Niemann-Pick C1 Protein. Am. J. Pathol. 166: 499-510 [Abstract] [Full Text]  
• Lehotzky, A., Tirian, L., Tokesi, N., Lenart, P., Szabo, B., Kovacs, J., Ovadi, J. (2004). Dynamic targeting of microtubules by TPPP/p25 affects cell survival. J. Cell Sci. 117: 6249-6259 [Abstract] [Full Text]  
• Albrecht, A. N., Kornak, U., Boddrich, A., Suring, K., Robinson, P. N., Stiege, A. C., Lurz, R., Stricker, S., Wanker, E. E., Mundlos, S. (2004). A molecular pathogenesis for transcription factor associated poly-alanine tract expansions. Hum Mol Genet 13: 2351-2359 [Abstract] [Full Text]  
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• Kelkar, S. A., Pfister, K. K., Crystal, R. G., Leopold, P. L. (2004). Cytoplasmic Dynein Mediates Adenovirus Binding to Microtubules. J. Virol. 78: 10122-10132 [Abstract] [Full Text]  
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• Maglova, L. M., Crowe, W. E., Russell, J. M. (2004). Perinuclear localization of Na-K-Cl-cotransporter protein after human cytomegalovirus infection. Am. J. Physiol. Cell Physiol. 286: C1324-C1334 [Abstract] [Full Text]  
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• Nollen, E. A. A., Garcia, S. M., van Haaften, G., Kim, S., Chavez, A., Morimoto, R. I., Plasterk, R. H. A. (2004). From The Cover: Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proc. Natl. Acad. Sci. USA 101: 6403-6408 [Abstract] [Full Text]  
• Fiebiger, E., Hirsch, C., Vyas, J. M., Gordon, E., Ploegh, H. L., Tortorella, D. (2004). Dissection of the Dislocation Pathway for Type I Membrane Proteins with a New Small Molecule Inhibitor, Eeyarestatin. Mol. Biol. Cell 15: 1635-1646 [Abstract] [Full Text]  
• Yanagisawa, M., Kaverina, I. N., Wang, A., Fujita, Y., Reynolds, A. B., Anastasiadis, P. Z. (2004). A Novel Interaction between Kinesin and p120 Modulates p120 Localization and Function. J. Biol. Chem. 279: 9512-9521 [Abstract] [Full Text]  
• Tanaka, M., Kim, Y. M., Lee, G., Junn, E., Iwatsubo, T., Mouradian, M. M. (2004). Aggresomes Formed by {alpha}-Synuclein and Synphilin-1 Are Cytoprotective. J. Biol. Chem. 279: 4625-4631 [Abstract] [Full Text]  
• Wojcik, C., Yano, M., DeMartino, G. N. (2004). RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis. J. Cell Sci. 117: 281-292 [Abstract] [Full Text]  
• Muqit, M. M.K., Davidson, S. M., Payne Smith, M. D., MacCormac, L. P., Kahns, S., Jensen, P. H., Wood, N. W., Latchman, D. S. (2004). Parkin is recruited into aggresomes in a stress-specific manner: over-expression of parkin reduces aggresome formation but can be dissociated from parkin's effect on neuronal survival. Hum Mol Genet 13: 117-135 [Abstract] [Full Text]  
• Becker, K. P., Hannun, Y. A. (2003). cPKC-dependent Sequestration of Membrane-recycling Components in a Subset of Recycling Endosomes. J. Biol. Chem. 278: 52747-52754 [Abstract] [Full Text]  
• Meriin, A. B., Zhang, X., Miliaras, N. B., Kazantsev, A., Chernoff, Y. O., McCaffery, J. M., Wendland, B., Sherman, M. Y. (2003). Aggregation of Expanded Polyglutamine Domain in Yeast Leads to Defects in Endocytosis. Mol. Cell. Biol. 23: 7554-7565 [Abstract] [Full Text]  
• Ardley, H. C., Scott, G. B., Rose, S. A., Tan, N. G. S., Markham, A. F., Robinson, P. A. (2003). Inhibition of Proteasomal Activity Causes Inclusion Formation in Neuronal and Non-Neuronal Cells Overexpressing Parkin. Mol. Biol. Cell 14: 4541-4556 [Abstract] [Full Text]  
• Zhao, J., Ren, Y., Jiang, Q., Feng, J. (2003). Parkin is recruited to the centrosome in response to inhibition of proteasomes. J. Cell Sci. 116: 4011-4019 [Abstract] [Full Text]  
• Grieshaber, S. S., Grieshaber, N. A., Hackstadt, T. (2003). Chlamydia trachomatis uses host cell dynein to traffic to the microtubule-organizing center in a p50 dynamitin-independent process. J. Cell Sci. 116: 3793-3802 [Abstract] [Full Text]  
• Petit, C., Giron, M.-L., Tobaly-Tapiero, J., Bittoun, P., Real, E., Jacob, Y., Tordo, N., de The, H., Saib, A. (2003). Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8. J. Cell Sci. 116: 3433-3442 [Abstract] [Full Text]  
• Debure, L., Vayssiere, J.-L., Rincheval, V., Loison, F., Le Drean, Y., Michel, D. (2003). Intracellular clusterin causes juxtanuclear aggregate formation and mitochondrial alteration. J. Cell Sci. 116: 3109-3121 [Abstract] [Full Text]  
• Chavez Zobel, A. T., Loranger, A., Marceau, N., Theriault, J. R., Lambert, H., Landry, J. (2003). Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G {alpha}B-crystallin mutant. Hum Mol Genet 12: 1609-1620 [Abstract] [Full Text]  
• Hatakeyama, S., Nakayama, K. I. (2003). Ubiquitylation as a Quality Control System for Intracellular Proteins. J Biochem 134: 1-8 [Abstract] [Full Text]  
• Hung, C.-F., Cheng, W.-F., He, L., Ling, M., Juang, J., Lin, C.-T., Wu, T-C. (2003). Enhancing Major Histocompatibility Complex Class I Antigen Presentation by Targeting Antigen to Centrosomes. Cancer Res. 63: 2393-2398 [Abstract] [Full Text]  
• Taylor, J. P., Tanaka, F., Robitschek, J., Sandoval, C. M., Taye, A., Markovic-Plese, S., Fischbeck, K. H. (2003). Aggresomes protect cells by enhancing the degradation of toxic polyglutamine-containing protein. Hum Mol Genet 12: 749-757 [Abstract] [Full Text]  
• Efimov, V. P. (2003). Roles of NUDE and NUDF Proteins of Aspergillus nidulans: Insights from Intracellular Localization and Overexpression Effects. Mol. Biol. Cell 14: 871-888 [Abstract] [Full Text]  
• Gautreau, A., Fievet, B. T., Brault, E., Antony, C., Houdusse, A., Louvard, D., Arpin, M. (2003). Isolation and Characterization of an Aggresome Determinant in the NF2 Tumor Suppressor. J. Biol. Chem. 278: 6235-6242 [Abstract] [Full Text]  
• Heath, C. M., Windsor, M., Wileman, T. (2003). Membrane Association Facilitates the Correct Processing of pp220 during Production of the Major Matrix Proteins of African Swine Fever Virus. J. Virol. 77: 1682-1690 [Abstract] [Full Text]  
• Keitel, V., Nies, A. T., Brom, M., Hummel-Eisenbeiss, J., Spring, H., Keppler, D. (2003). A common Dubin-Johnson syndrome mutation impairs protein maturation and transport activity of MRP2 (ABCC2). Am. J. Physiol. Gastrointest. Liver Physiol. 284: G165-G174 [Abstract] [Full Text]  
• Tanaka, Y., Ueda, K., Ozawa, T., Sakuragawa, N., Yokota, S., Sato, R., Okamura, S., Morita, M., Imanaka, T. (2002). Intracellular Accumulation of Antithrombin Morioka (C95R), a Novel Mutation Causing Type I Antithrombin Deficiency. J. Biol. Chem. 277: 51058-51067 [Abstract] [Full Text]  
• Lee, H.-J., Lee, S.-J. (2002). Characterization of Cytoplasmic alpha -Synuclein Aggregates. FIBRIL FORMATION IS TIGHTLY LINKED TO THE INCLUSION-FORMING PROCESS IN CELLS. J. Biol. Chem. 277: 48976-48983 [Abstract] [Full Text]  
• Gosavi, N., Lee, H.-J., Lee, J. S., Patel, S., Lee, S.-J. (2002). Golgi Fragmentation Occurs in the Cells with Prefibrillar alpha -Synuclein Aggregates and Precedes the Formation of Fibrillar Inclusion. J. Biol. Chem. 277: 48984-48992 [Abstract] [Full Text]  
• Junn, E., Lee, S. S., Suhr, U. T., Mouradian, M. M. (2002). Parkin Accumulation in Aggresomes Due to Proteasome Impairment. J. Biol. Chem. 277: 47870-47877 [Abstract] [Full Text]  
• Sodeik, B. (2002). Unchain my heart, baby let me go--the entry and intracellular transport of HIV. J. Cell Biol. 159: 393-395 [Abstract] [Full Text]  
• Verhoef, L. G.G.C., Lindsten, K., Masucci, M. G., Dantuma, N. P. (2002). Aggregate formation inhibits proteasomal degradation of polyglutamine proteins. Hum Mol Genet 11: 2689-2700 [Abstract] [Full Text]  
• Nakamichi, I., Hatakeyama, S., Nakayama, K. I. (2002). Formation of Mallory Body-like Inclusions and Cell Death Induced by Deregulated Expression of Keratin 18. Mol. Biol. Cell 13: 3441-3451 [Abstract] [Full Text]  
• Milewski, M. I., Mickle, J. E., Forrest, J. K., Stanton, B. A., Cutting, G. R. (2002). Aggregation of Misfolded Proteins Can Be a Selective Process Dependent upon Peptide Composition. J. Biol. Chem. 277: 34462-34470 [Abstract] [Full Text]  
• Dohner, K., Wolfstein, A., Prank, U., Echeverri, C., Dujardin, D., Vallee, R., Sodeik, B. (2002). Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport. Mol. Biol. Cell 13: 2795-2809 [Abstract] [Full Text]  
• Saliba, R. S., Munro, P. M. G., Luthert, P. J., Cheetham, M. E. (2002). The cellular fate of mutant rhodopsin: quality control, degradation and aggresome formation. J. Cell Sci. 115: 2907-2918 [Abstract] [Full Text]  
• Chadwick, B. P., Willard, H. F. (2002). Cell cycle-dependent localization of macroH2A in chromatin of the inactive X chromosome. J. Cell Biol. 157: 1113-1123 [Abstract] [Full Text]  
• Meriin, A. B., Zhang, X., He, X., Newnam, G. P., Chernoff, Y. O., Sherman, M. Y. (2002). Huntingtin toxicity in yeast model depends on polyglutamine aggregation mediated by a prion-like protein Rnq1. J. Cell Biol. 157: 997-1004 [Abstract] [Full Text]  
• Ganesh, S., Delgado-Escueta, A. V., Suzuki, T., Francheschetti, S., Riggio, C., Avanzini, G., Rabinowicz, A., Bohlega, S., Bailey, J., Alonso, M. E., Rasmussen, A., Thomson, A. E., Ochoa, A., Prado, A. J., Medina, M. T., Yamakawa, K. (2002). Genotype-phenotype correlations for EPM2A mutations in Lafora's progressive myoclonus epilepsy: exon 1 mutations associate with an early-onset cognitive deficit subphenotype. Hum Mol Genet 11: 1263-1271 [Abstract] [Full Text]  
• VanSlyke, J. K., Musil, L. S. (2002). Dislocation and degradation from the ER are regulated by cytosolic stress. J. Cell Biol. 157: 381-394 [Abstract] [Full Text]  
• Eystathioy, T., Chan, E. K. L., Tenenbaum, S. A., Keene, J. D., Griffith, K., Fritzler, M. J. (2002). A Phosphorylated Cytoplasmic Autoantigen, GW182, Associates with a Unique Population of Human mRNAs within Novel Cytoplasmic Speckles. Mol. Biol. Cell 13: 1338-1351 [Abstract] [Full Text]  
• Das Sarma, J., Meyer, R. A., Wang, F., Abraham, V., Lo, C. W., Koval, M. (2002). Multimeric connexin interactions prior to the trans-Golgi network. J. Cell Sci. 114: 4013-4024 [Abstract] [Full Text]  
• Lee, H.-J., Shin, S. Y., Choi, C., Lee, Y. H., Lee, S.-J. (2002). Formation and Removal of alpha -Synuclein Aggregates in Cells Exposed to Mitochondrial Inhibitors. J. Biol. Chem. 277: 5411-5417 [Abstract] [Full Text]  
• Tanudji, M., Hevi, S., Chuck, S. L. (2002). Improperly folded green fluorescent protein is secreted via a non-classical pathway. J. Cell Sci. 115: 3849-3857 [Abstract] [Full Text]  
• Maselli, A. G., Davis, R., Furukawa, R., Fechheimer, M. (2002). Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking protein. J. Cell Sci. 115: 1939-1949 [Abstract] [Full Text]  
• Manabe, R.-i., Kovalenko, M., Webb, D. J., Horwitz, A. R. (2002). GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115: 1497-1510 [Abstract] [Full Text]  
• Rajan, R. S., Illing, M. E., Bence, N. F., Kopito, R. R. (2001). Specificity in intracellular protein aggregation and inclusion body formation. Proc. Natl. Acad. Sci. USA 10.1073/pnas.181479798v1 [Abstract] [Full Text]  
• Fan, X., Dion, P., Laganiere, J., Brais, B., Rouleau, G. A. (2001). Oligomerization of polyalanine expanded PABPN1 facilitates nuclear protein aggregation that is associated with cell death. Hum Mol Genet 10: 2341-2351 [Abstract] [Full Text]  
• Nollen, E. A. A., Salomons, F. A., Brunsting, J. F., van der Want, J. J. L., Sibon, O. C. M., Kampinga, H. H. (2001). Dynamic changes in the localization of thermally unfolded nuclear proteins associated with chaperone-dependent protection. Proc. Natl. Acad. Sci. USA 10.1073/pnas.201112398v1 [Abstract] [Full Text]  
• Bence, N. F., Sampat, R. M., Kopito, R. R. (2001). Impairment of the Ubiquitin-Proteasome System by Protein Aggregation. Science 292: 1552-1555 [Abstract] [Full Text]  
• Meriin, A. B., Mabuchi, K., Gabai, V. L., Yaglom, J. A., Kazantsev, A., Sherman, M. Y. (2001). Intracellular Aggregation of Polypeptides with Expanded Polyglutamine Domain Is Stimulated by Stress-activated Kinase MEKK1. J. Cell Biol. 153: 851-864 [Abstract] [Full Text]  
• Heath, C. M., Windsor, M., Wileman, T. (2001). Aggresomes Resemble Sites Specialized for Virus Assembly. J. Cell Biol. 153: 449-456 [Abstract] [Full Text]  
• Gao, Y.-s., Sztul, E. (2001). A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton. J. Cell Biol. 152: 877-894 [Abstract] [Full Text]  
• Kabore, A., Wang, W., Russo, S., Beers, M. (2001). Biosynthesis of surfactant protein C: characterization of aggresome formation by EGFP chimeras containing propeptide mutants lacking conserved cysteine residues. J. Cell Sci. 114: 293-302 [Abstract]  
• INGANO, L.A. M., LENTINI, K. M., KOVACS, I., TANZI, R. E., KOVACS, D. M. (2000). Cytoplasmic Presenilin Aggregates in Proteasome Inhibitor-treated Cells. Ann. N. Y. Acad. Sci. 920: 259-260 [Full Text]  
• Pogacic, V., Dragon, F., Filipowicz, W. (2000). Human H/ACA Small Nucleolar RNPs and Telomerase Share Evolutionarily Conserved Proteins NHP2 and NOP10. Mol. Cell. Biol. 20: 9028-9040 [Abstract] [Full Text]  

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