The cis-Acting RNA Trafficking Signal from Myelin Basic Protein mRNA and Its Cognate trans-Acting Ligand hnRNP A2 Enhance Cap-dependent Translation

doi:10.1083/jcb.147.2.247

PeproTech: Your source for Cell Biology Research Reagents

This Article

	Full Text
	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kwon, S.
	Articles by Carson, J. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kwon, S.
	Articles by Carson, J. H.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/1999/10/247/ $5.00
The Journal of Cell Biology, Volume 147, Number 2, October 18, 1999 247-256

Original Article

The cis-Acting RNA Trafficking Signal from Myelin Basic Protein mRNA and Its Cognate trans-Acting Ligand hnRNP A2 Enhance Cap-dependent Translation

Sunjong Kwon^a, Elisa Barbarese^b, and John H. Carson^a
^a Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030
^b Department of Neurology, University of Connecticut Health Center, Farmington, Connecticut 06030

Correspondence to: John H. Carson, Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030. Tel:(860) 679-2130 Fax:(860) 679-3408 E-mail:jcarson{at}nso2.uchc.edu.

The 21 nucleotide RNA trafficking signal (RTS), originally identifiedin myelin basic protein mRNA, but also found in a variety ofother localized RNAs, is necessary and sufficient for transportof RNA along microtubules in oligodendrocytes. The RTS bindsspecifically to the RNA binding protein, hnRNP A2. Together,the RTS and hnRNP A2 comprise cis/trans determinants for severalsteps in the RNA trafficking pathway. Here we show that insertionof the RTS into green fluorescent protein (GFP) RNA enhancestranslation without affecting stability of microinjected RNA.In dicistronic RNA, the RTS enhances cap-dependent translationwithout affecting internal ribosome entry site (IRES)-dependenttranslation. The translation enhancer function of the RTS isposition, copy number, and cell type independent, hnRNP A2 dependent,and saturable with increasing amounts of injected RNA. Thisrepresents one of the first specific translation enhancer elementsidentified in a mammalian system.

Key Words: translation enhancer, hnRNP A2, RNA trafficking signal, dicistronicRNA, eIF4E

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Nelson, M. R., Luo, H., Vari, H. K., Cox, B. J., Simmonds, A. J., Krause, H. M., Lipshitz, H. D., Smibert, C. A. (2007). A Multiprotein Complex That Mediates Translational Enhancement in Drosophila. J. Biol. Chem. 282: 34031-34038 [Abstract] [Full Text]
Francone, V. P., Maggipinto, M. J., Kosturko, L. D., Barbarese, E. (2007). The Microtubule-Associated Protein Tumor Overexpressed Gene/Cytoskeleton-Associated Protein 5 Is Necessary for Myelin Basic Protein Expression in Oligodendrocytes. J. Neurosci. 27: 7654-7662 [Abstract] [Full Text]
Suprenant, K. A., Bloom, N., Fang, J., Lushington, G. (2007). The major vault protein is related to the toxic anion resistance protein (TelA) family. J. Exp. Biol. 210: 946-955 [Abstract] [Full Text]
Kosturko, L. D., Maggipinto, M. J., Korza, G., Lee, J. W., Carson, J. H., Barbarese, E. (2006). Heterogeneous Nuclear Ribonucleoprotein (hnRNP) E1 Binds to hnRNP A2 and Inhibits Translation of A2 Response Element mRNAs. Mol. Biol. Cell 17: 3521-3533 [Abstract] [Full Text]
Mickleburgh, I., Chabanon, H., Nury, D., Fan, K., Burtle, B., Chrzanowska-Lightowlers, Z., Hesketh, J. (2006). Elongation factor 1{alpha} binds to the region of the metallothionein-1 mRNA implicated in perinuclear localization--importance of an internal stem-loop. RNA 12: 1397-1407 [Abstract] [Full Text]
Fahling, M., Mrowka, R., Steege, A., Martinka, P., Persson, P. B., Thiele, B. J. (2006). Heterogeneous Nuclear Ribonucleoprotein-A2/B1 Modulate Collagen Prolyl 4-Hydroxylase, {alpha} (I) mRNA Stability. J. Biol. Chem. 281: 9279-9286 [Abstract] [Full Text]
Iwahashi, C. K., Yasui, D. H., An, H.-J., Greco, C. M., Tassone, F., Nannen, K., Babineau, B., Lebrilla, C. B., Hagerman, R. J., Hagerman, P. J. (2006). Protein composition of the intranuclear inclusions of FXTAS. Brain 129: 256-271 [Abstract] [Full Text]
Kosturko, L. D., Maggipinto, M. J., D'Sa, C., Carson, J. H., Barbarese, E. (2005). The Microtubule-associated Protein Tumor Overexpressed Gene Binds to the RNA Trafficking Protein Heterogeneous Nuclear Ribonucleoprotein A2. Mol. Biol. Cell 16: 1938-1947 [Abstract] [Full Text]
Moran-Jones, K., Wayman, L., Kennedy, D. D., Reddel, R. R., Sara, S., Snee, M. J., Smith, R. (2005). hnRNP A2, a potential ssDNA/RNA molecular adapter at the telomere. Nucleic Acids Res 33: 486-496 [Abstract] [Full Text]
Beriault, V., Clement, J.-F., Levesque, K., LeBel, C., Yong, X., Chabot, B., Cohen, E. A., Cochrane, A. W., Rigby, W. F. C., Mouland, A. J. (2004). A Late Role for the Association of hnRNP A2 with the HIV-1 hnRNP A2 Response Elements in Genomic RNA, Gag, and Vpr Localization. J. Biol. Chem. 279: 44141-44153 [Abstract] [Full Text]
RYDER, S. P., WILLIAMSON, J. R. (2004). Specificity of the STAR/GSG domain protein Qk1: Implications for the regulation of myelination. RNA 10: 1449-1458 [Abstract] [Full Text]
Shan, J., Munro, T. P., Barbarese, E., Carson, J. H., Smith, R. (2003). A Molecular Mechanism for mRNA Trafficking in Neuronal Dendrites. J. Neurosci. 23: 8859-8866 [Abstract] [Full Text]
Huang, Y.-S., Carson, J. H., Barbarese, E., Richter, J. D. (2003). Facilitation of dendritic mRNA transport by CPEB. Genes Dev. 17: 638-653 [Abstract] [Full Text]
Wang, H., Iacoangeli, A., Popp, S., Muslimov, I. A., Imataka, H., Sonenberg, N., Lomakin, I. B., Tiedge, H. (2002). Dendritic BC1 RNA: Functional Role in Regulation of Translation Initiation. J. Neurosci. 22: 10232-10241 [Abstract] [Full Text]
Back, S. H., Shin, S., Jang, S. K. (2002). Polypyrimidine Tract-binding Proteins Are Cleaved by Caspase-3 during Apoptosis. J. Biol. Chem. 277: 27200-27209 [Abstract] [Full Text]
Ma, A. S. W., Moran-Jones, K., Shan, J., Munro, T. P., Snee, M. J., Hoek, K. S., Smith, R. (2002). Heterogeneous Nuclear Ribonucleoprotein A3, a Novel RNA Trafficking Response Element-binding Protein. J. Biol. Chem. 277: 18010-18020 [Abstract] [Full Text]
Percipalle, P., Jonsson, A., Nashchekin, D., Karlsson, C., Bergman, T., Guialis, A., Daneholt, B. (2002). Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins. Nucleic Acids Res 30: 1725-1734 [Abstract] [Full Text]
Aronov, S., Aranda, G., Behar, L., Ginzburg, I. (2001). Axonal Tau mRNA Localization Coincides with Tau Protein in Living Neuronal Cells and Depends on Axonal Targeting Signal. J. Neurosci. 21: 6577-6587 [Abstract] [Full Text]
Mouland, A. J., Xu, H., Cui, H., Krueger, W., Munro, T. P., Prasol, M., Mercier, J., Rekosh, D., Smith, R., Barbarese, E., Cohen, E. A., Carson, J. H. (2001). RNA Trafficking Signals in Human Immunodeficiency Virus Type 1. Mol. Cell. Biol. 21: 2133-2143 [Abstract] [Full Text]
Pederson, T. (2001). Fluorescent RNA cytochemistry: tracking gene transcripts in living cells. Nucleic Acids Res 29: 1013-1016 [Abstract] [Full Text]
Shan, J., Moran-Jones, K., Munro, T. P., Kidd, G. J., Winzor, D. J., Hoek, K. S., Smith, R. (2000). Binding of an RNA Trafficking Response Element to Heterogeneous Nuclear Ribonucleoproteins A1 and A2. J. Biol. Chem. 275: 38286-38295 [Abstract] [Full Text]
Kawamura, H., Tomozoe, Y., Akagi, T., Kamei, D., Ochiai, M., Yamada, M. (2002). Identification of the Nucleocytoplasmic Shuttling Sequence of Heterogeneous Nuclear Ribonucleoprotein D-like Protein JKTBP and Its Interaction with mRNA. J. Biol. Chem. 277: 2732-2739 [Abstract] [Full Text]