Original Article

Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast

Correspondence to: Yoshinori Ohsumi, Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan. Tel:81-564-55-7515 Fax:81-564-55-7516 E-mail:yohsumi{at}nibb.ac.jp.

We characterized Apg8/Aut7p essential for autophagy in yeast. Apg8p was transcriptionally upregulated in response to starvation and mostly existed as a protein bound to membrane under both growing and starvation conditions. Immunofluorescence microscopy revealed that the intracellular localization of Apg8p changed drastically after shift to starvation. Apg8p resided on unidentified tiny dot structures dispersed in the cytoplasm at growing phase. During starvation, it was localized on large punctate structures, some of which were confirmed to be autophagosomes and autophagic bodies by immuno-EM. Besides these structures, we found that Apg8p was enriched on isolation membranes and in electron less-dense regions, which should contain Apg8p-localized membrane- or lipid-containing structures. These structures would represent intermediate structures during autophagosome formation. Here, we also showed that microtubule does not play an essential role in the autophagy in yeast. The result does not match with the previously proposed role of Apg8/Aut7p, delivery of autophagosome to the vacuole along microtubule. Moreover, it is revealed that autophagosome formation is severely impaired in the apg8 null mutant. Apg8p would play an important role in the autophagosome formation.

Key Words: autophagy, autophagosome, Apg8/Aut7p, Saccharomyces cerevisiae, vacuole

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• Melendez, A., Talloczy, Z., Seaman, M., Eskelinen, E.-L., Hall, D. H., Levine, B. (2003). Autophagy Genes Are Essential for Dauer Development and Life-Span Extension in C. elegans. Science 301: 1387-1391 [Abstract] [Full Text]  
• He, H., Dang, Y., Dai, F., Guo, Z., Wu, J., She, X., Pei, Y., Chen, Y., Ling, W., Wu, C., Zhao, S., Liu, J. O., Yu, L. (2003). Post-translational Modifications of Three Members of the Human MAP1LC3 Family and Detection of a Novel Type of Modification for MAP1LC3B. J. Biol. Chem. 278: 29278-29287 [Abstract] [Full Text]  
• Brown, C. R., Liu, J., Hung, G.-C., Carter, D., Cui, D., Chiang, H.-L. (2003). The Vid Vesicle to Vacuole Trafficking Event Requires Components of the SNARE Membrane Fusion Machinery. J. Biol. Chem. 278: 25688-25699 [Abstract] [Full Text]  
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• Scherz-Shouval, R., Sagiv, Y., Shorer, H., Elazar, Z. (2003). The COOH Terminus of GATE-16, an Intra-Golgi Transport Modulator, Is Cleaved by the Human Cysteine Protease HsApg4A. J. Biol. Chem. 278: 14053-14058 [Abstract] [Full Text]  
• Dementhon, K., Paoletti, M., Pinan-Lucarre, B., Loubradou-Bourges, N., Sabourin, M., Saupe, S. J., Clave, C. (2003). Rapamycin Mimics the Incompatibility Reaction in the Fungus Podospora anserina. Eukaryot Cell 2: 238-246 [Abstract] [Full Text]  
• Reggiori, F., Wang, C.-W., Stromhaug, P. E., Shintani, T., Klionsky, D. J. (2003). Vps51 Is Part of the Yeast Vps Fifty-three Tethering Complex Essential for Retrograde Traffic from the Early Endosome and Cvt Vesicle Completion. J. Biol. Chem. 278: 5009-5020 [Abstract] [Full Text]  
• Abeliovich, H., Zhang, C., Dunn, W. A. Jr., Shokat, K. M., Klionsky, D. J. (2003). Chemical Genetic Analysis of Apg1 Reveals A Non-kinase Role in the Induction of Autophagy. Mol. Biol. Cell 14: 477-490 [Abstract] [Full Text]  
• Doelling, J. H., Walker, J. M., Friedman, E. M., Thompson, A. R., Vierstra, R. D. (2002). The APG8/12-activating Enzyme APG7 Is Required for Proper Nutrient Recycling and Senescence in Arabidopsis thaliana. J. Biol. Chem. 277: 33105-33114 [Abstract] [Full Text]  
• Wurmser, A. E., Emr, S. D. (2002). Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy. J. Cell Biol. 158: 761-772 [Abstract] [Full Text]  
• Tanida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., Kominami, E. (2002). Human Apg3p/Aut1p Homologue Is an Authentic E2 Enzyme for Multiple Substrates, GATE-16, GABARAP, and MAP-LC3, and Facilitates the Conjugation of hApg12p to hApg5p. J. Biol. Chem. 277: 13739-13744 [Abstract] [Full Text]  
• Munafo, D. B., Colombo, M. I. (2002). A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation. J. Cell Sci. 114: 3619-3629 [Abstract] [Full Text]  
• Reggiori, F., Klionsky, D. J. (2002). Autophagy in the Eukaryotic Cell. Eukaryot Cell 1: 11-21 [Full Text]  
• Kim, J., Huang, W.-P., Stromhaug, P. E., Klionsky, D. J. (2002). Convergence of Multiple Autophagy and Cytoplasm to Vacuole Targeting Components to a Perivacuolar Membrane Compartment Prior to de Novo Vesicle Formation. J. Biol. Chem. 277: 763-773 [Abstract] [Full Text]  
• Guan, J., Stromhaug, P. E., George, M. D., Habibzadegah-Tari, P., Bevan, A., Dunn, W. A. Jr., Klionsky, D. J. (2001). Cvt18/Gsa12 Is Required for Cytoplasm-to-Vacuole Transport, Pexophagy, and Autophagy in Saccharomyces cerevisiae and Pichia pastoris. Mol. Biol. Cell 12: 3821-3838 [Abstract] [Full Text]  
• Ishihara, N., Hamasaki, M., Yokota, S., Suzuki, K., Kamada, Y., Kihara, A., Yoshimori, T., Noda, T., Ohsumi, Y. (2001). Autophagosome Requires Specific Early Sec Proteins for Its Formation and NSF/SNARE for Vacuolar Fusion. Mol. Biol. Cell 12: 3690-3702 [Abstract] [Full Text]  
• Abeliovich, H., Klionsky, D. J. (2001). Autophagy in Yeast: Mechanistic Insights and Physiological Function. Microbiol. Mol. Biol. Rev. 65: 463-479 [Abstract] [Full Text]  
• Mizushima, N., Yamamoto, A., Hatano, M., Kobayashi, Y., Kabeya, Y., Suzuki, K., Tokuhisa, T., Ohsumi, Y., Yoshimori, T. (2001). Dissection of Autophagosome Formation using Apg5-deficient Mouse Embryonic Stem Cells. J. Cell Biol. 152: 657-668 [Abstract] [Full Text]  
• Kihara, A., Noda, T., Ishihara, N., Ohsumi, Y. (2001). Two Distinct Vps34 Phosphatidylinositol 3-Kinase Complexes Function in Autophagy and Carboxypeptidase Y Sorting in Saccharomyces cerevisiae. J. Cell Biol. 152: 519-530 [Abstract] [Full Text]  
• Kim, J., Huang, W.-P., Klionsky, D. J. (2001). Membrane Recruitment of Aut7p in the Autophagy and Cytoplasm to Vacuole Targeting Pathways Requires Aut1p, Aut2p, and the Autophagy Conjugation Complex. J. Cell Biol. 152: 51-64 [Abstract] [Full Text]  
• Klionsky, D. J., Emr, S. D. (2000). Autophagy as a Regulated Pathway of Cellular Degradation. Science 290: 1717-1721 [Abstract] [Full Text]  
• Abeliovich, H., Dunn, W. A. , Jr., Kim, J., Klionsky, D. J. (2000). Dissection of Autophagosome Biogenesis into Distinct Nucleation and Expansion Steps. J. Cell Biol. 151: 1025-1034 [Abstract] [Full Text]  
• Sattler, T., Mayer, A. (2000). Cell-free Reconstitution of Microautophagic Vacuole Invagination and Vesicle Formation. J. Cell Biol. 151: 529-538 [Abstract] [Full Text]  
• Kirisako, T., Ichimura, Y., Okada, H., Kabeya, Y., Mizushima, N., Yoshimori, T., Ohsumi, M., Takao, T., Noda, T., Ohsumi, Y. (2000). The Reversible Modification Regulates the Membrane-Binding State of Apg8/Aut7 Essential for Autophagy and the Cytoplasm to Vacuole Targeting Pathway. J. Cell Biol. 151: 263-276 [Abstract] [Full Text]  
• Brown, C. R., McCann, J. A., Chiang, H.-L. (2000). The Heat Shock Protein Ssa2p Is Required for Import of Fructose-1,6-Bisphosphatase into Vid Vesicles. J. Cell Biol. 150: 65-76 [Abstract] [Full Text]  
• George, M. D., Baba, M., Scott, S. V., Mizushima, N., Garrison, B. S., Ohsumi, Y., Klionsky, D. J. (2000). Apg5p Functions in the Sequestration Step in the Cytoplasm-to-Vacuole Targeting and Macroautophagy Pathways. Mol. Biol. Cell 11: 969-982 [Abstract] [Full Text]  
• Huang, W.-P., Scott, S. V., Kim, J., Klionsky, D. J. (2000). The Itinerary of a Vesicle Component, Aut7p/Cvt5p, Terminates in the Yeast Vacuole via the Autophagy/Cvt Pathways. J. Biol. Chem. 275: 5845-5851 [Abstract] [Full Text]  
• Noda, T., Kim, J., Huang, W.-P., Baba, M., Tokunaga, C., Ohsumi, Y., Klionsky, D. J. (2000). Apg9p/Cvt7p Is an Integral Membrane Protein Required for Transport Vesicle Formation in the Cvt and Autophagy Pathways. J. Cell Biol. 148: 465-480 [Abstract] [Full Text]  
• Paz, Y., Elazar, Z., Fass, D. (2000). Structure of GATE-16, Membrane Transport Modulator and Mammalian Ortholog of Autophagocytosis Factor Aut7p. J. Biol. Chem. 275: 25445-25450 [Abstract] [Full Text]  
• Tanida, I., Tanida-Miyake, E., Ueno, T., Kominami, E. (2001). The Human Homolog of Saccharomyces cerevisiae Apg7p Is a Protein-activating Enzyme for Multiple Substrates Including Human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem. 276: 1701-1706 [Abstract] [Full Text]  
• Legesse-Miller, A., Sagiv, Y., Glozman, R., Elazar, Z. (2000). Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes. J. Biol. Chem. 275: 32966-32973 [Abstract] [Full Text]  
• Komatsu, M., Tanida, I., Ueno, T., Ohsumi, M., Ohsumi, Y., Kominami, E. (2001). The C-terminal Region of an Apg7p/Cvt2p Is Required for Homodimerization and Is Essential for Its E1 Activity and E1-E2 Complex Formation. J. Biol. Chem. 276: 9846-9854 [Abstract] [Full Text]  
• Chan, T.-F., Bertram, P. G., Ai, W., Zheng, X. F. S. (2001). Regulation of APG14 Expression by the GATA-type Transcription Factor Gln3p. J. Biol. Chem. 276: 6463-6467 [Abstract] [Full Text]  
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