Syndapin Isoforms Participate in Receptor-mediated Endocytosis and Actin Organization

doi:10.1083/jcb.148.5.1047

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© The Rockefeller University Press, 0021-9525/2000/3/1047/ $5.00
The Journal of Cell Biology, Volume 148, Number 5, March 6, 2000 1047-1062

Original Article

Syndapin Isoforms Participate in Receptor-mediated Endocytosis and Actin Organization

Britta Qualmann^a and Regis B. Kelly^a
^a Department of Biochemistry and Biophysics and the Hormone Research Institute, University of California, San Francisco, California 94143-0534

Correspondence to: Regis B. Kelly, Department of Biochemistry and Biophysics and the Hormone Research Institute, University of California, 513 Parnassus Avenue, 1090 HSW, San Francisco, CA 94143-0534. Tel:(415) 476-4095 Fax:(415) 731-3612 E-mail:rkelly{at}biochem.ucsf.edu.

Syndapin I (SdpI) interacts with proteins involved in endocytosisand actin dynamics and was therefore proposed to be a molecularlink between the machineries for synaptic vesicle recyclingand cytoskeletal organization. We here report the identificationand characterization of SdpII, a ubiquitously expressed isoformof the brain-specific SdpI. Certain splice variants of rat SdpIIin other species were named FAP52 and PACSIN 2. SdpII bindsdynamin I, synaptojanin, synapsin I, and the neural Wiskott-Aldrichsyndrome protein (N-WASP), a stimulator of Arp2/3 induced actinfilament nucleation. In neuroendocrine cells, SdpII colocalizeswith dynamin, consistent with a role for syndapin in dynamin-mediatedendocytic processes. The src homology 3 (SH3) domain of SdpIand -II inhibited receptor-mediated internalization of transferrin,demonstrating syndapin involvement in endocytosis in vivo. Overexpressionof full-length syndapins, but not the NH₂-terminal part or theSH3 domains alone, had a strong effect on cortical actin organizationand induced filopodia. This syndapin overexpression phenotypeappears to be mediated by the Arp2/3 complex at the cell peripherybecause it was completely suppressed by coexpression of a cytosolicCOOH-terminal fragment of N-WASP. Consistent with a role inactin dynamics, syndapins localized to sites of high actin turnover,such as filopodia tips and lamellipodia. Our results stronglysuggest that syndapins link endocytosis and actin dynamics.

Key Words: pheochromocytoma, Arp2/3 complex, dynamin, neural Wiskott-Aldrichsyndrome protein, filopodia

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