Fyn-binding Protein (Fyb)/SLP-76-associated Protein (SLAP), Ena/Vasodilator-stimulated Phosphoprotein (VASP) Proteins and the Arp2/3 Complex Link T Cell Receptor (TCR) Signaling to the Actin Cytoskeleton

doi:10.1083/jcb.149.1.181

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© The Rockefeller University Press, 0021-9525/2000/4/181/ $5.00
The Journal of Cell Biology, Volume 149, Number 1, April 3, 2000 181-194

Original Article

Fyn-binding Protein (Fyb)/SLP-76–associated Protein (SLAP), Ena/Vasodilator-stimulated Phosphoprotein (VASP) Proteins and the Arp2/3 Complex Link T Cell Receptor (TCR) Signaling to the Actin Cytoskeleton

Matthias Krause^a, Antonio S. Sechi^a, Marlies Konradt^a, David Monner^a, Frank B. Gertler^b, and Jürgen Wehland^a
^a Department of Cell Biology, Gesellschaft für Biotechnologische Forschung (GBF), D-38124 Braunschweig, Germany
^b Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02138-4307

Correspondence to: Jürgen Wehland, Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany. Tel:49-531-6181-415 Fax:49-531-6181-444 E-mail:jwe{at}gbf.de.

T cell receptor (TCR)-driven activation of helper T cells inducesa rapid polarization of their cytoskeleton towards bound antigenpresenting cells (APCs). We have identified the Fyn- and SLP-76–associatedprotein Fyb/SLAP as a new ligand for Ena/ vasodilator-stimulatedphosphoprotein (VASP) homology 1 (EVH1) domains. Upon TCR engagement,Fyb/SLAP localizes at the interface between T cells and anti-CD3–coatedbeads, where Evl, a member of the Ena/VASP family, Wiskott-Aldrichsyndrome protein (WASP) and the Arp2/3 complex are also found.In addition, Fyb/SLAP is restricted to lamellipodia of spreadingplatelets. In activated T cells, Fyb/SLAP associates with Ena/VASPfamily proteins and is present within biochemical complexescontaining WASP, Nck, and SLP-76. Inhibition of binding betweenFyb/SLAP and Ena/VASP proteins or WASP and the Arp2/3 compleximpairs TCR-dependent actin rearrangement, suggesting that theseinteractions play a key role in linking T cell signaling toremodeling of the actin cytoskeleton.

Key Words: Evl, EVH1, WASP, Nck, actin cytoskeleton

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