JCB logo
BioLegend: Antibody Reagents
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kano, F.
Right arrow Articles by Murata, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kano, F.
Right arrow Articles by Murata, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2000/4/357/ $5.00
The Journal of Cell Biology, Volume 149, Number 2, April 17, 2000 357-368

Original Article

MEK and Cdc2 Kinase Are Sequentially Required for Golgi Disassembly in MDCK Cells by the Mitotic Xenopus Extracts

Fumi Kanoa,b,d, Katsuya Takenakab, Akitsugu Yamamotoc, Kuniaki Nagayamaa, Eisuke Nishidab, and Masayuki Murataa,d
a Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
b Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-Ku, Kyoto 606-8502, Japan
c Department of Physiology, Kansai Medical University, Osaka 570-8506, Japan
d CREST, Japan Science and Technology Corporation, Japan

Correspondence to: Masayuki Murata, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan. Tel:181-564-55-7815 Fax:181-564-52-7913 E-mail:mmurata{at}nips.ac.jp.

At the onset of mitosis, the Golgi apparatus, which consists of several cisternae, disperses throughout the cell to be partitioned into daughter cells. The molecular mechanisms of this process are now beginning to be understood. To investigate the biochemical requirements and kinetics of mitotic Golgi membrane dynamics in polarized cells, we have reconstituted the disassembly of the Golgi apparatus by introducing Xenopus egg extracts into permeabilized Mardin-Darby canine kidney (MDCK) cells. We used green fluorescence protein (GFP)-tagged galactosyltransferase-expressing MDCK cells to analyze the morphological changes of the Golgi membrane in the semi-intact system. Analyses by fluorescence and electron microscopies showed that the Golgi disassembly can be dissected into two elementary processes morphologically. In the first process, the perinuclear Golgi stacks break into punctate structures, intermediates, which are comprised of mini-stacks of cisternae associating with apical microtubule networks. In the second process, the structures fragment more thoroughly or substantially relocate to the ER. Our analyses further showed that cdc2 kinase and mitogen-activated protein kinase kinase (MAPKK = MEK) are differently involved in these two processes: the first process is mainly regulated by MEK and the second mainly by cdc2.

Key Words: Golgi complex, mitosis, semi-intact cells, GFP, kinase


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents