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The Journal of Cell Biology, Vol 15, 579-588, Copyright © 1962 by Rockefeller University Press

ARTICLE

N-VINYLPYRROLIDONE AS A WATER COMPATIBLE CONSTITUENT OF EMBEDDING RESINS FOR SECTIONING IN ELECTRON MICROSCOPY

A. C. Fabergé D.Sc.1 and C. Willard Lewis Jr. 1

1 From the Genetics Foundation and The Electron Microscope Laboratory, University of Texas, Austin.

Mr. Lewis' present address is The Mercy Institute for Biomedical Research, Denver Colorado

The use as an embedding resin for ultrathin sectioning of a cross-linked triple copolymer of N-vinylpyrrolidone, acrylonitrile, and ethylene glycol dimethacrylate is described. The first of these components is miscible with water, in all proportions, and can be used as a dehydrating agent, or, alternatively, ethanol may be used in the standard way. Polymerization is carried out at 37°C or even lower temperatures. This resin is unsuitable for use after osmium fixatives, but after permanganates it gives results similar to epoxy. Photographs of rye root-tip cells fixed in permanganate and sectioned in this resin are presented. Because of the water-permeable nature of the product and low polymerization temperature, this resin appears to have possibilities for histochemistry.

Submitted on June 15, 1962


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