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Published online 7 August 2000. doi:10.1083/jcb.150.3.499
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© The Rockefeller University Press, 0021-9525/2000/8/499/ $5.00
The Journal of Cell Biology, Volume 150, Number 3, August 7, 2000 499-512

Original Article

A Kinesin-related Protein, KRP180, Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division

Gregory C. Rogersa, Kitty K. Chuia, Edwin W. Leea, Karen P. Wedamana, David J. Sharpa, Gina Hollanda, Robert L. Morrisa, and Jonathan M. Scholeya
a Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616

Correspondence to: Jonathan M. Scholey, Section of Molecular and Cellular Biology, 149 Briggs Hall, University of California at Davis, Davis, CA 95616. Tel:(530) 752-2271 Fax:(530) 752-7522 E-mail:jmscholey{at}ucdavis.edu.

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP180, in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP180 concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP180 is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Key Words: Xklp2, kinesin, sea urchin, mitosis, {gamma}-tubulin


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