|
|
|
|
|
|
|
||
Original Article |
Correspondence to: Stefan Stamm, Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany. Tel:49 89 8578 3625 Fax:49 89 8578 3749 E-mail:stefan{at}stamms-lab.net.
The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug- induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as 
-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.
Key Words: subnuclear compartments, transcription, cell cycle, MCF7 differentiation, actinomycin D
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
