Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes

doi:10.1083/jcb.150.5.989

Published online 5 September 2000. doi:10.1083/jcb.150.5.989

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© The Rockefeller University Press, 0021-9525/2000/9/989/ $5.00
The Journal of Cell Biology, Volume 150, Number 5, September 4, 2000 989-1000

Original Article

Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes

Yosuke Takei^a, Junlin Teng^a, Akihiro Harada^a, and Nobutaka Hirokawa^a
^a Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Correspondence to: Nobutaka Hirokawa, Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Tel:81-3-5841-3326 Fax:81-3-5802-8646 E-mail:hirokawa{at}m.u-tokyo.ac.jp.

Tau and MAP1B are the main members of neuronal microtubule-associatedproteins (MAPs), the functions of which have remained obscurebecause of a putative functional redundancy (Harada, A., K.Oguchi, S. Okabe, J. Kuno, S. Terada, T. Ohshima, R. Sato-Yoshitake,Y. Takei, T. Noda, and N. Hirokawa. 1994. Nature. 369:488–491;Takei, Y., S. Kondo, A. Harada, S. Inomata, T. Noda, and N.Hirokawa. 1997. J. Cell Biol. 137:1615–1626). To unmaskthe role of these proteins, we generated double-knockout micewith disrupted tau and map1b genes and compared their phenotypeswith those of single-knockout mice. In the analysis of micewith a genetic background of predominantly C57Bl/6J, a hypoplasticcommissural axon tract and disorganized neuronal layering wereobserved in the brains of the tau+/+map1b-/- mice. These phenotypesare markedly more severe in tau-/-map1b-/- double mutants, indicatingthat tau and MAP1B act in a synergistic fashion. Primary culturesof hippocampal neurons from tau-/-map1b-/- mice showed inhibitedaxonal elongation. In these cells, a generation of new axonsvia bundling of microtubules at the neck of the growth conesappeared to be disturbed. Cultured cerebellar neurons from tau-/-map1b-/-mice showed delayed neuronal migration concomitant with suppressedneurite elongation. These findings indicate the cooperativefunctions of tau and MAP1B in vivo in axonal elongation andneuronal migration as regulators of microtubule organization.

Key Words: tau, MAP1B, axon, growth cone, neuronal migration

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