Tor-mediated Induction of Autophagy Via an Apg1 Protein Kinase Complex

doi:10.1083/jcb.150.6.1507

Epitomics: The Rabbit Monoclonal Company

Published online 18 September 2000. doi:10.1083/jcb.150.6.1507

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© The Rockefeller University Press, 0021-9525/2000/9/1507/ $5.00
The Journal of Cell Biology, Volume 150, Number 6, September 18, 2000 1507-1513

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Tor-mediated Induction of Autophagy Via an Apg1 Protein Kinase Complex

Yoshiaki Kamada^a,b, Tomoko Funakoshi^a, Takahiro Shintani^a, Kazuya Nagano^c, Mariko Ohsumi^c, and Yoshinori Ohsumi^a,b
^a Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
^b School of Life Science, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan
^c Department of Biosciences, Teikyo University of Science and Technology, Yamanashi 409-0193, Japan

Correspondence to: Yoshinori Ohsumi, Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan. Tel:81-564-55-7515 Fax:81-564-55-7516

Autophagy is a membrane trafficking to vacuole/lysosome inducedby nutrient starvation. In Saccharomyces cerevisiae, Tor protein,a phosphatidylinositol kinase-related kinase, is involved inthe repression of autophagy induction by a largely unknown mechanism.Here, we show that the protein kinase activity of Apg1 is enhancedby starvation or rapamycin treatment. In addition, we have alsofound that Apg13, which binds to and activates Apg1, is hyperphosphorylatedin a Tor-dependent manner, reducing its affinity to Apg1. ThisApg1–Apg13 association is required for autophagy, butnot for the cytoplasm-to-vacuole targeting (Cvt) pathway, anothervesicular transport mechanism in which factors essential forautophagy (Apg proteins) are also employed under vegetativegrowth conditions. Finally, other Apg1-associating proteins,such as Apg17 and Cvt9, are shown to function specifically inautophagy or the Cvt pathway, respectively, suggesting thatthe Apg1 complex plays an important role in switching betweentwo distinct vesicular transport systems in a nutrient-dependentmanner.

Key Words: starvation, Cvt, yeast, rapamycin, phosphorylation

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