Ordering the Final Events in Yeast Exocytosis

doi:10.1083/jcb.151.2.439

Published online 18 October 2000. doi:10.1083/jcb.151.2.439

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© The Rockefeller University Press, 0021-9525/2000/10/439/ $5.00
The Journal of Cell Biology, Volume 151, Number 2, October 16, 2000 439-452

Original Article

Ordering the Final Events in Yeast Exocytosis

Eric Grote^a, Chavela M. Carr^a, and Peter J. Novick^a
^a Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520

Correspondence to: Peter J. Novick, Dept. Cell Biology, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8002. Tel:(203) 785-5871 Fax:(203) 785-7226

In yeast, assembly of exocytic soluble N-ethylmaleimide–sensitivefusion protein (NSF) attachment protein receptor (SNARE) complexesbetween the secretory vesicle SNARE Sncp and the plasma membraneSNAREs Ssop and Sec9p occurs at a late stage of the exocyticreaction. Mutations that block either secretory vesicle deliveryor tethering prevent SNARE complex assembly and the localizationof Sec1p, a SNARE complex binding protein, to sites of secretion.By contrast, wild-type levels of SNARE complexes persist inthe sec1-1 mutant after a secretory block is imposed, suggestinga role for Sec1p after SNARE complex assembly. In the sec18-1mutant, cis-SNARE complexes containing surface-accessible Sncpaccumulate in the plasma membrane. Thus, one function of Sec18pis to disassemble SNARE complexes on the postfusion membrane.

Key Words: NSF, membrane fusion, SNAREs, exocyst, Sec1

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