The Transcription Coactivator CBP is a Dynamic Component of the Promyelocytic Leukemia Nuclear Body

doi:10.1083/jcb.152.5.1099

Published online 5 March 2001. doi:10.1083/jcb.152.5.1099

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© The Rockefeller University Press, 0021-9525/2001/3/1099/ $5.00
The Journal of Cell Biology, Volume 152, Number 5, March 5, 2001 1099-1106

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The Transcription Coactivator CBP is a Dynamic Component of the Promyelocytic Leukemia Nuclear Body

François-Michel Boisvert^a, Michael J. Kruhlak^a, Alan K. Box^a, Michael J. Hendzel^b, and David P. Bazett-Jones^a
^a Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta T2N 4N1, Canada
^b Cross Cancer Institute, University of Alberta, Edmonton, Alberta T6G 1Z2, Canada

Correspondence to: David P. Bazett-Jones, Department of Cell Biology and Anatomy, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB, T2N 4N1 Canada. Tel:(403) 220-3025 Fax:(403) 270-0737 E-mail:bazett{at}ucalgary.ca.

The transcription coactivator and histone acetyltransferaseCAMP response element–binding protein (CBP) has been demonstratedto accumulate in promyelocytic leukemia (PML) bodies. We showthat this accumulation is cell type specific. In cells whereCBP does not normally accumulate in PML bodies, it can be inducedto accumulate in PML bodies through overexpression of eitherCBP or Pml, but not Sp100. Using fluorescence recovery afterphotobleaching, we demonstrate that CBP moves rapidly into andout of PML bodies. In contrast, Pml and Sp100 are relativelyimmobile in the nucleoplasm and within PML nuclear bodies. Theypossess the characteristics expected of proteins that wouldplay a structural role in the integrity of these subnucleardomains. Our results are consistent with CBP being a dynamiccomponent of PML bodies and that the steady-state level in thesestructures can be modulated by Pml.

Key Words: nuclear structure, promyelocytic leukemia, PML body, ND10, fluorescencerecovery after photobleaching

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