The Golgi-associated Hook3 Protein Is a Member of a Novel Family of Microtubule-binding Proteins

doi:10.1083/jcb.152.5.923

Published online 26 February 2001. doi:10.1083/jcb.152.5.923

This Article

	Full Text
	PDF (Full Text)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Walenta, J. H.
	Articles by Krämer, H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Walenta, J. H.
	Articles by Krämer, H.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2001/3/923/ $5.00
The Journal of Cell Biology, Volume 152, Number 5, March 5, 2001 923-934

Original Article

The Golgi-associated Hook3 Protein Is a Member of a Novel Family of Microtubule-binding Proteins

Jason H. Walenta^a, Aaron J. Didier^a, Xinran Liu^a, and Helmut Krämer^a
^a Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390

Correspondence to: Helmut Krämer, Center for Basic Neuroscience and Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9111. Tel:(214) 648-1860 Fax:(214) 648-1801 E-mail:kramer{at}utsw.swmed.edu.

Microtubules are central to the spatial organization of diversemembrane-trafficking systems. Here, we report that Hook proteinsconstitute a novel family of cytosolic coiled coil proteinsthat bind to organelles and to microtubules. The conserved NH₂-terminaldomains of Hook proteins mediate attachment to microtubules,whereas the more divergent COOH-terminal domains mediate thebinding to organelles. Human Hook3 bound to Golgi membranesin vitro and was enriched in the cis-Golgi in vivo. Unlike othercis-Golgi–associated proteins, however, a large fractionof Hook3 maintained its juxtanuclear localization after BrefeldinA treatment, indicating a Golgi-independent mechanism for Hook3localization. Because overexpression of Hook3 caused fragmentationof the Golgi complex, we propose that Hook3 participates indefining the architecture and localization of the mammalianGolgi complex.

Key Words: membrane trafficking, Hook protein, endosomes, brefeldin A,Golgi complex

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Shim, S. Y., Wang, J., Asada, N., Neumayer, G., Tran, H. C., Ishiguro, K.-i., Sanada, K., Nakatani, Y., Nguyen, M. D. (2008). Protein 600 Is a Microtubule/Endoplasmic Reticulum-Associated Protein in CNS Neurons. J. Neurosci. 28: 3604-3614 [Abstract] [Full Text]
Ciampi, R., Giordano, T. J, Wikenheiser-Brokamp, K., Koenig, R. J, Nikiforov, Y. E (2007). HOOK3-RET: a novel type of RET/PTC rearrangement in papillary thyroid carcinoma. Endocr Relat Cancer 14: 445-452 [Abstract] [Full Text]
Sano, H., Ishino, M., Kramer, H., Shimizu, T., Mitsuzawa, H., Nishitani, C., Kuroki, Y. (2007). The Microtubule-binding Protein Hook3 Interacts with a Cytoplasmic Domain of Scavenger Receptor A. J. Biol. Chem. 282: 7973-7981 [Abstract] [Full Text]
Kim, H.-S., Takahashi, M., Matsuo, K., Ono, Y. (2007). Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex. GENES CELLS 12: 421-434 [Abstract] [Full Text]
Kama, R., Robinson, M., Gerst, J. E. (2007). Btn2, a Hook1 Ortholog and Potential Batten Disease-Related Protein, Mediates Late Endosome-Golgi Protein Sorting in Yeast. Mol. Cell. Biol. 27: 605-621 [Abstract] [Full Text]
Wilhelmsen, K., Ketema, M., Truong, H., Sonnenberg, A. (2006). KASH-domain proteins in nuclear migration, anchorage and other processes. J. Cell Sci. 119: 5021-5029 [Abstract] [Full Text]
Broers, J. L. V., Ramaekers, F. C. S., Bonne, G., Yaou, R. B., Hutchison, C. J. (2006). Nuclear lamins: laminopathies and their role in premature ageing.. Physiol. Rev. 86: 967-1008 [Abstract] [Full Text]
Sztul, E., Lupashin, V. (2006). Role of tethering factors in secretory membrane traffic. Am. J. Physiol. Cell Physiol. 290: C11-C26 [Abstract] [Full Text]
Pulipparacharuvil, S., Akbar, M. A., Ray, S., Sevrioukov, E. A., Haberman, A. S., Rohrer, J., Kramer, H. (2005). Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. J. Cell Sci. 118: 3663-3673 [Abstract] [Full Text]
Lin, C.-M., Chen, H.-J., Leung, C. L., Parry, D. A. D., Liem, R. K. H. (2005). Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex. J. Cell Sci. 118: 3727-3738 [Abstract] [Full Text]
Nelson, D. E., Virok, D. P., Wood, H., Roshick, C., Johnson, R. M., Whitmire, W. M., Crane, D. D., Steele-Mortimer, O., Kari, L., McClarty, G., Caldwell, H. D. (2005). Chlamydial IFN-{gamma} immune evasion is linked to host infection tropism. Proc. Natl. Acad. Sci. USA 102: 10658-10663 [Abstract] [Full Text]
Hermann, G. J., Schroeder, L. K., Hieb, C. A., Kershner, A. M., Rabbitts, B. M., Fonarev, P., Grant, B. D., Priess, J. R. (2005). Genetic Analysis of Lysosomal Trafficking in Caenorhabditis elegans. Mol. Biol. Cell 16: 3273-3288 [Abstract] [Full Text]
Le-Niculescu, H., Niesman, I., Fischer, T., DeVries, L., Farquhar, M. G. (2005). Identification and Characterization of GIV, a Novel G{alpha}i/s -interacting Protein Found on COPI, Endoplasmic Reticulum-Golgi Transport Vesicles. J. Biol. Chem. 280: 22012-22020 [Abstract] [Full Text]
Yoshino, A., Setty, S. R. G., Poynton, C., Whiteman, E. L., Saint-Pol, A., Burd, C. G., Johannes, L., Holzbaur, E. L., Koval, M., McCaffery, J. M., Marks, M. S. (2005). tGolgin-1 (p230, golgin-245) modulates Shiga-toxin transport to the Golgi and Golgi motility towards the microtubule-organizing centre. J. Cell Sci. 118: 2279-2293 [Abstract] [Full Text]
Vedrenne, C., Klopfenstein, D. R., Hauri, H.-P. (2005). Phosphorylation Controls CLIMP-63-mediated Anchoring of the Endoplasmic Reticulum to Microtubules. Mol. Biol. Cell 16: 1928-1937 [Abstract] [Full Text]
Farah, C. A., Liazoghli, D., Perreault, S., Desjardins, M., Guimont, A., Anton, A., Lauzon, M., Kreibich, G., Paiement, J., Leclerc, N. (2005). Interaction of Microtubule-associated Protein-2 and p63: A NEW LINK BETWEEN MICROTUBULES AND ROUGH ENDOPLASMIC RETICULUM MEMBRANES IN NEURONS. J. Biol. Chem. 280: 9439-9449 [Abstract] [Full Text]
Lee, J. S.H., Chang, M. I., Tseng, Y., Wirtz, D. (2005). Cdc42 Mediates Nucleus Movement and MTOC Polarization in Swiss 3T3 Fibroblasts under Mechanical Shear Stress. Mol. Biol. Cell 16: 871-880 [Abstract] [Full Text]
Wruck, C. J., Funke-Kaiser, H., Pufe, T., Kusserow, H., Menk, M., Schefe, J. H., Kruse, M. L., Stoll, M., Unger, T. (2005). Regulation of Transport of the Angiotensin AT2 Receptor by a Novel Membrane-Associated Golgi Protein. Arterioscler. Thromb. Vasc. Bio. 25: 57-64 [Abstract] [Full Text]
Luiro, K., Yliannala, K., Ahtiainen, L., Maunu, H., Jarvela, I., Kyttala, A., Jalanko, A. (2004). Interconnections of CLN3, Hook1 and Rab proteins link Batten disease to defects in the endocytic pathway. Hum Mol Genet 13: 3017-3027 [Abstract] [Full Text]
Fischer, J. A., Acosta, S., Kenny, A., Cater, C., Robinson, C., Hook, J. (2004). Drosophila Klarsicht Has Distinct Subcellular Localization Domains for Nuclear Envelope and Microtubule Localization in the Eye. Genetics 168: 1385-1393 [Abstract] [Full Text]
Whited, J. L., Cassell, A., Brouillette, M., Garrity, P. A. (2004). Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons. Development 131: 4677-4686 [Abstract] [Full Text]
Larocca, M. C., Shanks, R. A., Tian, L., Nelson, D. L., Stewart, D. M., Goldenring, J. R. (2004). AKAP350 Interaction with cdc42 Interacting Protein 4 at the Golgi Apparatus. Mol. Biol. Cell 15: 2771-2781 [Abstract] [Full Text]
Kaiser, F., Kaufmann, S. H. E., Zerrahn, J. (2004). IIGP, a member of the IFN inducible and microbial defense mediating 47 kDa GTPase family, interacts with the microtubule binding protein hook3. J. Cell Sci. 117: 1747-1756 [Abstract] [Full Text]
Richardson, S. C. W., Winistorfer, S. C., Poupon, V., Luzio, J. P., Piper, R. C. (2004). Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton. Mol. Biol. Cell 15: 1197-1210 [Abstract] [Full Text]
Kim, J., Parker, J. S. L., Murray, K. E., Nibert, M. L. (2004). Nucleoside and RNA Triphosphatase Activities of Orthoreovirus Transcriptase Cofactor {micro}2. J. Biol. Chem. 279: 4394-4403 [Abstract] [Full Text]
Andrade, J., Zhao, H., Titus, B., Timm Pearce, S., Barroso, M. (2004). The EF-Hand Ca2+-binding Protein p22 Plays a Role in Microtubule and Endoplasmic Reticulum Organization and Dynamics with Distinct Ca2+-binding Requirements. Mol. Biol. Cell 15: 481-496 [Abstract] [Full Text]
Spitzenberger, F., Pietropaolo, S., Verkade, P., Habermann, B., Lacas-Gervais, S., Mziaut, H., Pietropaolo, M., Solimena, M. (2003). Islet Cell Autoantigen of 69 kDa Is an Arfaptin-related Protein Associated with the Golgi Complex of Insulinoma INS-1 Cells. J. Biol. Chem. 278: 26166-26173 [Abstract] [Full Text]
Mendoza-Lujambio, I., Burfeind, P., Dixkens, C., Meinhardt, A., Hoyer-Fender, S., Engel, W., Neesen, J. (2002). The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse. Hum Mol Genet 11: 1647-1658 [Abstract] [Full Text]
Perez, F., Pernet-Gallay, K., Nizak, C., Goodson, H. V., Kreis, T. E., Goud, B. (2002). CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family. J. Cell Biol. 156: 631-642 [Abstract] [Full Text]
Perez, F., Pernet-Gallay, K., Nizak, C., Goodson, H. V., Kreis, T. E., Goud, B. (2002). CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family. J. Cell Biol. 156: 631-642 [Abstract] [Full Text]