FtsZ Ring Formation at the Chloroplast Division Site in Plants

doi:10.1083/jcb.153.1.111

Published online 2 April 2001. doi:10.1083/jcb.153.1.111

This Article

	Full Text
	PDF (Full Text)
	Supplemental Material Index
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Vitha, S.
	Articles by Osteryoung, K. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Vitha, S.
	Articles by Osteryoung, K. W.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2001/4/111/ $5.00
The Journal of Cell Biology, Volume 153, Number 1, April 2, 2001 111-120

Original Article

FtsZ Ring Formation at the Chloroplast Division Site in Plants

Stanislav Vitha^a, Rosemary S. McAndrew^a, and Katherine W. Osteryoung^a
^a Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824

Correspondence to: Katherine W. Osteryoung, Department of Plant Biology, 166 Plant Biology Building, Michigan State University, East Lansing, MI 48824. Tel:(517) 355-4685 Fax:(517) 353-1926 E-mail:osteryou{at}msu.edu.

Among the events that accompanied the evolution of chloroplastsfrom their endosymbiotic ancestors was the host cell recruitmentof the prokaryotic cell division protein FtsZ to function inchloroplast division. FtsZ, a structural homologue of tubulin,mediates cell division in bacteria by assembling into a ringat the midcell division site. In higher plants, two nuclear-encodedforms of FtsZ, FtsZ1 and FtsZ2, play essential and functionallydistinct roles in chloroplast division, but whether this involvesring formation at the division site has not been determinedpreviously. Using immunofluorescence microscopy and expressionof green fluorescent protein fusion proteins in Arabidopsisthaliana, we demonstrate here that FtsZ1 and FtsZ2 localizeto coaligned rings at the chloroplast midpoint. Antibodies specificfor recognition of FtsZ1 or FtsZ2 proteins in Arabidopsis alsorecognize related polypeptides and detect midplastid rings inpea and tobacco, suggesting that midplastid ring formation byFtsZ1 and FtsZ2 is universal among flowering plants. Perturbationin the level of either protein in transgenic plants is accompaniedby plastid division defects and assembly of FtsZ1 and FtsZ2into filaments and filament networks not observed in wild-type,suggesting that previously described FtsZ-containing cytoskeletal-likenetworks in chloroplasts may be artifacts of FtsZ overexpression.

Key Words: plastids, organelle replication, organelle fission, cytoskeleton,mitochondria

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Fujiwara, M. T., Hashimoto, H., Kazama, Y., Abe, T., Yoshida, S., Sato, N., Itoh, R. D. (2008). The Assembly of the FtsZ Ring at the Mid-Chloroplast Division Site Depends on a Balance Between the Activities of AtMinE1 and ARC11/AtMinD1. Plant Cell Physiol 49: 345-361 [Abstract] [Full Text]
Maple, J., Moller, S. G. (2007). Interdependency of formation and localisation of the Min complex controls symmetric plastid division. J. Cell Sci. 120: 3446-3456 [Abstract] [Full Text]
Yoder, D. W., Kadirjan-Kalbach, D., Olson, B. J. S. C., Miyagishima, S.-y., DeBlasio, S. L., Hangarter, R. P., Osteryoung, K. W. (2007). Effects of Mutations in Arabidopsis FtsZ1 on Plastid Division, FtsZ Ring Formation and Positioning, and FtsZ Filament Morphology in Vivo. Plant Cell Physiol 48: 775-791 [Abstract] [Full Text]
Maple, J., Moller, S. G. (2007). Plastid Division: Evolution, Mechanism and Complexity. ANN BOT (LOND) 99: 565-579 [Abstract] [Full Text]
Lopez-Juez, E. (2007). Plastid biogenesis, between light and shadows. J Exp Bot 58: 11-26 [Abstract] [Full Text]
Miyagishima, S.-y., Froehlich, J. E., Osteryoung, K. W. (2006). PDV1 and PDV2 Mediate Recruitment of the Dynamin-Related Protein ARC5 to the Plastid Division Site. Plant Cell 18: 2517-2530 [Abstract] [Full Text]
Lohse, S., Hause, B., Hause, G., Fester, T. (2006). FtsZ Characterization and Immunolocalization in the Two Phases of Plastid Reorganization in Arbuscular Mycorrhizal Roots of Medicago truncatula. Plant Cell Physiol 47: 1124-1134 [Abstract] [Full Text]
Balmer, Y., Vensel, W. H, DuPont, F. M, Buchanan, B. B, Hurkman, W. J (2006). Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability. J Exp Bot 57: 1591-1602 [Abstract] [Full Text]
Aldridge, C., Moller, S. G. (2005). The Plastid Division Protein AtMinD1 Is a Ca2+-ATPase Stimulated by AtMinE1. J. Biol. Chem. 280: 31673-31678 [Abstract] [Full Text]
Vaishnava, S., Morrison, D. P., Gaji, R. Y., Murray, J. M., Entzeroth, R., Howe, D. K., Striepen, B. (2005). Plastid segregation and cell division in the apicomplexan parasite Sarcocystis neurona. J. Cell Sci. 118: 3397-3407 [Abstract] [Full Text]
Aldridge, C., Maple, J., Moller, S. G. (2005). The molecular biology of plastid division in higher plants. J Exp Bot 56: 1061-1077 [Abstract] [Full Text]
Natesan, S. K. A., Sullivan, J. A., Gray, J. C. (2005). Stromules: a characteristic cell-specific feature of plastid morphology. J Exp Bot 56: 787-797 [Abstract] [Full Text]
Shimada, H., Koizumi, M., Kuroki, K., Mochizuki, M., Fujimoto, H., Ohta, H., Masuda, T., Takamiya, K.-i. (2004). ARC3, a Chloroplast Division Factor, is a Chimera of Prokaryotic FtsZ and Part of Eukaryotic Phosphatidylinositol-4-phosphate 5-kinase. Plant Cell Physiol 45: 960-967 [Abstract] [Full Text]
Nishida, K., Misumi, O., Yagisawa, F., Kuroiwa, H., Nagata, T., Kuroiwa, T. (2004). Triple Immunofluorescent Labeling of FtsZ, Dynamin, and EF-Tu Reveals a Loose Association Between the Inner and Outer Membrane Mitochondrial Division Machinery in the Red Alga Cyanidioschyzon merolae. J. Histochem. Cytochem. 52: 843-849 [Abstract] [Full Text]
Raynaud, C., Cassier-Chauvat, C., Perennes, C., Bergounioux, C. (2004). An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division. Plant Cell 16: 1801-1811 [Abstract] [Full Text]
Fujiwara, M. T., Nakamura, A., Itoh, R., Shimada, Y., Yoshida, S., Moller, S. G. (2004). Chloroplast division site placement requires dimerization of the ARC11/AtMinD1 protein in Arabidopsis. J. Cell Sci. 117: 2399-2410 [Abstract] [Full Text]
Ferguson, P. L., Shaw, G. S. (2004). Human S100B Protein Interacts with the Escherichia coli Division Protein FtsZ in a Calcium-sensitive Manner. J. Biol. Chem. 279: 18806-18813 [Abstract] [Full Text]
Osteryoung, K. W., Nunnari, J. (2003). The Division of Endosymbiotic Organelles. Science 302: 1698-1704 [Abstract] [Full Text]
Gilson, P. R., Yu, X.-C., Hereld, D., Barth, C., Savage, A., Kiefel, B. R., Lay, S., Fisher, P. R., Margolin, W., Beech, P. L. (2003). Two Dictyostelium Orthologs of the Prokaryotic Cell Division Protein FtsZ Localize to Mitochondria and Are Required for the Maintenance of Normal Mitochondrial Morphology. Eukaryot Cell 2: 1315-1326 [Abstract] [Full Text]
Gonzalez, J. M., Jimenez, M., Velez, M., Mingorance, J., Andreu, J. M., Vicente, M., Rivas, G. (2003). Essential Cell Division Protein FtsZ Assembles into One Monomer-thick Ribbons under Conditions Resembling the Crowded Intracellular Environment. J. Biol. Chem. 278: 37664-37671 [Abstract] [Full Text]
Small, E., Addinall, S. G. (2003). Dynamic FtsZ polymerization is sensitive to the GTP to GDP ratio and can be maintained at steady state using a GTP-regeneration system. Microbiology 149: 2235-2242 [Abstract] [Full Text]
Vitha, S., Froehlich, J. E., Koksharova, O., Pyke, K. A., van Erp, H., Osteryoung, K. W. (2003). ARC6 Is a J-Domain Plastid Division Protein and an Evolutionary Descendant of the Cyanobacterial Cell Division Protein Ftn2. Plant Cell 15: 1918-1933 [Abstract] [Full Text]
Gao, H., Kadirjan-Kalbach, D., Froehlich, J. E., Osteryoung, K. W. (2003). From the Cover: ARC5, a cytosolic dynamin-like protein from plants, is part of the chloroplast division machinery. Proc. Natl. Acad. Sci. USA 100: 4328-4333 [Abstract] [Full Text]
Miyagishima, S.-y., Nishida, K., Mori, T., Matsuzaki, M., Higashiyama, T., Kuroiwa, H., Kuroiwa, T. (2003). A Plant-Specific Dynamin-Related Protein Forms a Ring at the Chloroplast Division Site. Plant Cell 15: 655-665 [Abstract] [Full Text]
Ding, Z., Zhao, Z., Jakubowski, S. J., Krishnamohan, A., Margolin, W., Christie, P. J. (2002). A Novel Cytology-Based, Two-Hybrid Screen for Bacteria Applied to Protein-Protein Interaction Studies of a Type IV Secretion System. J. Bacteriol. 184: 5572-5582 [Abstract] [Full Text]
Fulgosi, H., Gerdes, L., Westphal, S., Glockmann, C., Soll, J. (2002). Cell and chloroplast division requires ARTEMIS. Proc. Natl. Acad. Sci. USA 99: 11501-11506 [Abstract] [Full Text]
Arimura, S.-i., Tsutsumi, N. (2002). A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division. Proc. Natl. Acad. Sci. USA 99: 5727-5731 [Abstract] [Full Text]
Itoh, R., Fujiwara, M., Nagata, N., Yoshida, S. (2001). A Chloroplast Protein Homologous to the Eubacterial Topological Specificity Factor MinE Plays a Role in Chloroplast Division. Plant Physiol. 127: 1644-1655 [Abstract] [Full Text]
McAndrew, R. S., Froehlich, J. E., Vitha, S., Stokes, K. D., Osteryoung, K. W. (2001). Colocalization of Plastid Division Proteins in the Chloroplast Stromal Compartment Establishes a New Functional Relationship between FtsZ1 and FtsZ2 in Higher Plants. Plant Physiol. 127: 1656-1666 [Abstract] [Full Text]
Miyagishima, S.-y., Takahara, M., Mori, T., Kuroiwa, H., Higashiyama, T., Kuroiwa, T. (2001). Plastid Division Is Driven by a Complex Mechanism That Involves Differential Transition of the Bacterial and Eukaryotic Division Rings. Plant Cell 13: 2257-2268 [Abstract] [Full Text]