A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae

doi:10.1083/jcb.153.1.47

Published online 26 March 2001. doi:10.1083/jcb.153.1.47

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© The Rockefeller University Press, 0021-9525/2001/4/47/ $5.00
The Journal of Cell Biology, Volume 153, Number 1, April 2, 2001 47-62

Original Article

A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae

Olivia W. Rossanese^a, Catherine A. Reinke^a, Brooke J. Bevis^a, Adam T. Hammond^a, Irina B. Sears^a, James O'Connor^a, and Benjamin S. Glick^a
^a Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637

Correspondence to: Benjamin S. Glick, Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th St., Chicago, IL 60637. Tel:(773) 702-5315 Fax:(773) 702-3172 E-mail:bsglick{at}midway.uchicago.edu.

In Saccharomyces cerevisiae, Golgi elements are present in thebud very early in the cell cycle. We have analyzed this Golgiinheritance process using fluorescence microscopy and genetics.In rapidly growing cells, late Golgi elements show an actin-dependentconcentration at sites of polarized growth. Late Golgi elementsare apparently transported into the bud along actin cables andare also retained in the bud by a mechanism that may involveactin. A visual screen for mutants defective in the inheritanceof late Golgi elements yielded multiple alleles of CDC1. Mutationsin CDC1 severely depolarize the actin cytoskeleton, and thesemutations prevent late Golgi elements from being retained inthe bud. The efficient localization of late Golgi elements tothe bud requires the type V myosin Myo2p, further suggestingthat actin plays a role in Golgi inheritance. Surprisingly,early and late Golgi elements are inherited by different pathways,with early Golgi elements localizing to the bud in a Cdc1p-and Myo2p-independent manner. We propose that early Golgi elementsarise from ER membranes that are present in the bud. These twopathways of Golgi inheritance in S. cerevisiae resemble Golgiinheritance pathways in vertebrate cells.

Key Words: Golgi apparatus, endoplasmic reticulum, budding yeast, actins,myosin

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