Circulating Skeletal Stem Cells

doi:10.1083/jcb.153.5.1133

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Published online 29 May 2001. doi:10.1083/jcb.153.5.1133

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© The Rockefeller University Press, 0021-9525/2001/5/1133/ $5.00
The Journal of Cell Biology, Volume 153, Number 5, May 28, 2001 1133-1140

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Circulating Skeletal Stem Cells

Sergei A. Kuznetsov^a, Mahesh H. Mankani^a, Stan Gronthos^a, Kazuhito Satomura^b, Paolo Bianco^c, and Pamela Gehron Robey^a
^a Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892
^b First Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Tokushima, Tokushima 770-8504, Japan
^c Dipartmento di Medicina Sperimental e Patologia, Universita "La Sapienza," Rome 00161, Italy

Correspondence to: Sergei A. Kuznetsov, Building 30, Room 228 30 Convent Drive MSC 4320 Bethesda, MD 20892. Tel:(301) 402-2476 Fax:(301) 402-0824 E-mail:skuznets{at}mail.nih.gov.

We report the isolation of adherent, clonogenic, fibroblast-likecells with osteogenic and adipogenic potential from the bloodof four mammalian species. These cells phenotypically resemblebut are distinguishable from skeletal stem cells found in bonemarrow (stromal stem cells, "mesenchymal stem cells"). The osteogenicpotential of the blood-borne cells was proven by an in vivotransplantation assay in which either polyclonal or single colony–derivedstrains were transplanted into the subcutis of immunocompromisedmice, and the donor origin of the fully differentiated bonecells was proven using species-specific probes. This is thefirst definitive proof of the existence of circulating skeletalstem cells in mammals.

Key Words: blood cells, tissue culture, adherent colonies, transplantation,osteogenesis

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