HIM-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes

doi:10.1083/jcb.153.6.1227

Published online 11 June 2001. doi:10.1083/jcb.153.6.1227

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© The Rockefeller University Press, 0021-9525/2001/6/1227/ $5.00
The Journal of Cell Biology, Volume 153, Number 6, June 11, 2001 1227-1238

Original Article

HIM-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes

Mary Howe^a, Kent L. McDonald^b, Donna G. Albertson^c, and Barbara J. Meyer^a
^a Howard Hughes Medical Institute, Department of Molecular and Cell Biology
^b Electron Microscope Laboratory, University of California, Berkeley, Berkeley, California 94720
^c Cancer Research Institute, University of California, San Francisco, San Francisco, California 94143

Correspondence to: Barbara J. Meyer, Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, Berkeley, CA 94720-3204. Tel:(510) 643-5585 Fax:(510) 643-5584 E-mail:bjmeyer{at}uclink4.berkeley.edu.

Macromolecular structures called kinetochores attach and movechromosomes within the spindle during chromosome segregation.Using electron microscopy, we identified a structure on theholocentric mitotic and meiotic chromosomes of Caenorhabditiselegans that resembles the mammalian kinetochore. This structurefaces the poles on mitotic chromosomes but encircles meioticchromosomes. Worm kinetochores require the evolutionarily conservedHIM-10 protein for their structure and function. HIM-10 localizesto the kinetochores and mediates attachment of chromosomes tothe spindle. Depletion of HIM-10 disrupts kinetochore structure,causes a failure of bipolar spindle attachment, and resultsin chromosome nondisjunction. HIM-10 is related to the Nuf2kinetochore proteins conserved from yeast to humans. Thus, theextended kinetochores characteristic of C. elegans holocentricchromosomes provide a guide to the structure, molecular architecture,and function of conventional kinetochores.

Key Words: kinetochore, holocentric chromosomes, mitosis, meiosis, Caenorhabditiselegans

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