Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text PDF (Full Text) Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Adams, D. J. Articles by Rasko, J. E.J. Search for Related Content PubMed PubMed Citation Articles by Adams, D. J. Articles by Rasko, J. E.J. Social Bookmarking                      What's this?

¹ The University of Sydney, Basic & Clinical Genomics Laboratory, Department of Physiology and Institute for Biomedical Research
² Gene Therapy Research Unit, Centenary Institute of Cancer Medicine & Cell Biology and Sydney Cancer Centre, Royal Prince Alfred Hospital, Sydney, NSW 2006, Australia
³ University of Miami School of Medicine, Department of Biochemistry and Molecular Biology, Miami, FL 33136
⁴ University of Erlangen, Institute of Biochemistry, 91054 Erlangen, Germany

Address correspondence to Brian J. Morris, D.Sc., Basic & Clinical Genomics Laboratory, Department of Physiology and Institute for Biomedical Research, Bldg F13, The University of Sydney, NSW 2006, Australia. Tel.: 61-2-9351-3688. Fax: 61-2-9351-2227. E-mail: brianm{at}physiol.usyd.edu.au

The formation of the active spliceosome, its recruitment to active areas of transcription, and its role in pre-mRNA splicing depends on the association of a number of multifunctional serine/arginine-rich (SR) proteins. ZNF265 is an arginine/serine-rich (RS) domain containing zinc finger protein with conserved pre-mRNA splicing protein motifs. Here we show that ZNF265 immunoprecipitates from splicing extracts in association with mRNA, and that it is able to alter splicing patterns of Tra2-ß1 transcripts in a dose-dependent manner in HEK 293 cells. Yeast two-hybrid analysis and immunoprecipitation indicated interaction of ZNF265 with the essential splicing factor proteins U1-70K and U2AF³⁵. Confocal microscopy demonstrated colocalization of ZNF265 with the motor neuron gene product SMN, the snRNP protein U1-70K, the SR protein SC35, and with the transcriptosomal components p300 and YY1. Transfection of HT-1080 cells with ZNF265–EGFP fusion constructs showed that nuclear localization of ZNF265 required the RS domain. Alignment with other RS domain–containing proteins revealed a high degree of SR dipeptide conservation. These data show that ZNF265 functions as a novel component of the mRNA processing machinery.

Key Words: zinc finger protein; RS domain; SR proteins; RNA processing; nuclear localization; renin

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Mangs, A. H., Speirs, H. J.L., Goy, C., Adams, D. J., Markus, M. A., Morris, B. J. (2006). XE7: A novel splicing factor that interacts with ASF/SF2 and ZNF265. Nucleic Acids Res 34: 4976-4986 [Abstract] [Full Text]  
• Buratti, E., Brindisi, A., Giombi, M., Tisminetzky, S., Ayala, Y. M., Baralle, F. E. (2005). TDP-43 Binds Heterogeneous Nuclear Ribonucleoprotein A/B through Its C-terminal Tail: AN IMPORTANT REGION FOR THE INHIBITION OF CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR EXON 9 SPLICING. J. Biol. Chem. 280: 37572-37584 [Abstract] [Full Text]  
• Adams, D. J., van der Weyden, L., Gergely, F. V., Arends, M. J., Ng, B. L., Tannahill, D., Kanaar, R., Markus, A., Morris, B. J., Bradley, A. (2005). BRCTx Is a Novel, Highly Conserved RAD18-Interacting Protein. Mol. Cell. Biol. 25: 779-788 [Abstract] [Full Text]  
• Wang, C.-Y., Liang, Y.-J., Lin, Y.-S., Shih, H.-M., Jou, Y.-S., Yu, W. C. Y. (2004). YY1AP, A Novel Co-activator of YY1. J. Biol. Chem. 279: 17750-17755 [Abstract] [Full Text]  
• Sakashita, E., Tatsumi, S., Werner, D., Endo, H., Mayeda, A. (2004). Human RNPS1 and Its Associated Factors: a Versatile Alternative Pre-mRNA Splicing Regulator In Vivo. Mol. Cell. Biol. 24: 1174-1187 [Abstract] [Full Text]  
• Adams, D. J., Beveridge, D. J., van der Weyden, L., Mangs, H., Leedman, P. J., Morris, B. J. (2003). HADHB, HuR, and CP1 Bind to the Distal 3'-Untranslated Region of Human Renin mRNA and Differentially Modulate Renin Expression. J. Biol. Chem. 278: 44894-44903 [Abstract] [Full Text]  
• Plambeck, C. A., Kwan, A. H. Y., Adams, D. J., Westman, B. J., van der Weyden, L., Medcalf, R. L., Morris, B. J., Mackay, J. P. (2003). The Structure of the Zinc Finger Domain from Human Splicing Factor ZNF265 Fold. J. Biol. Chem. 278: 22805-22811 [Abstract] [Full Text]  
• Shomron, N., Malca, H., Vig, I., Ast, G. (2002). Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing. Nucleic Acids Res 30: 4127-4137 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents