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Published 23 July 2001. doi:10.1083/jcb.200104083
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© The Rockefeller University Press, 0021-9525/2001/7/293 $5.00
The Journal of Cell Biology, Volume 154, Number 2, July 23, 2001 293-308

Article

 Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product

Karen E. Tucker1,2, Maria Teresa Berciano3, Erica Y. Jacobs1,2, David F. LePage1, Karl B. Shpargel1,2, Jennifer J. Rossire1,2, Edward K.L. Chan4, Miguel Lafarga3, Ronald A. Conlon1 and A. Gregory Matera1,2

1 Department of Genetics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106
2 Program in Cell Biology, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106
3 Department of Anatomy and Cell Biology, Faculty of Medicine, University of Cantabria, 39011 Santander, Spain
4 W.M. Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, CA 92037

Address correspondence to Gregory Matera, Dept. of Genetics, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-4955. Tel.: (216) 368-4922. Fax: (216) 368-3432. E-mail: gxm26{at}po.cwru.edu

Cajal bodies (CBs) are nuclear suborganelles involved in the biogenesis of small nuclear ribonucleoproteins (snRNPs). In addition to snRNPs, they are highly enriched in basal transcription and cell cycle factors, the nucleolar proteins fibrillarin (Fb) and Nopp140 (Nopp), the survival motor neuron (SMN) protein complex, and the CB marker protein, p80 coilin. We report the generation of knockout mice lacking the COOH-terminal 487 amino acids of coilin. Northern and Western blot analyses demonstrate that we have successfully removed the full-length coilin protein from the knockout animals. Some homozygous mutant animals are viable, but their numbers are reduced significantly when crossed to inbred backgrounds. Analysis of tissues and cell lines from mutant animals reveals the presence of extranucleolar foci that contain Fb and Nopp but not other typical nucleolar markers. These so-called "residual" CBs neither condense Sm proteins nor recruit members of the SMN protein complex. Transient expression of wild-type mouse coilin in knockout cells results in formation of CBs and restores these missing epitopes. Our data demonstrate that full-length coilin is essential for proper formation and/or maintenance of CBs and that recruitment of snRNP and SMN complex proteins to these nuclear subdomains requires sequences within the coilin COOH terminus.

Key Words: coilin; SMN; SMA; snRNPs; nuclear organization


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