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Published 23 July 2001. doi:10.1083/jcb.200104073
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© The Rockefeller University Press, 0021-9525/2001/7/317 $5.00
The Journal of Cell Biology, Volume 154, Number 2, July 23, 2001 317-330

Article

 Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum

Eija Jokitalo1, Noemi Cabrera-Poch2, Graham Warren3 and David T. Shima4

1 Institute of Biotechnology, Electron Microscopy Unit, University of Helsinki, 00014 Helsinki, Finland
2 Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY 10032
3 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
4 Endothelial Cell Biology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK

Address correspondence to David T. Shima, Endothelial Cell Biology Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. Tel.: 44-207-269-2880. Fax: 44-207-269-3417. E-mail: d.shima{at}magic.lif.icnet.uk

We have examined the fate of Golgi membranes during mitotic inheritance in animal cells using four-dimensional fluorescence microscopy, serial section reconstruction of electron micrographs, and peroxidase cytochemistry to track the fate of a Golgi enzyme fused to horseradish peroxidase. All three approaches show that partitioning of Golgi membranes is mediated by Golgi clusters that persist throughout mitosis, together with shed vesicles that are often found associated with spindle microtubules. We have been unable to find evidence that Golgi membranes fuse during the later phases of mitosis with the endoplasmic reticulum (ER) as a strategy for Golgi partitioning (Zaal, K.J., C.L. Smith, R.S. Polishchuk, N. Altan, N.B. Cole, J. Ellenberg, K. Hirschberg, J.F. Presley, T.H. Roberts, E. Siggia, et al. 1999. Cell. 99:589–601) and suggest that these results, in part, are the consequence of slow or abortive folding of GFP–Golgi chimeras in the ER. Furthermore, we show that accurate partitioning is accomplished early in mitosis, by a process of cytoplasmic redistribution of Golgi fragments and vesicles yielding a balance of Golgi membranes on either side of the metaphase plate before cell division.

Key Words: Golgi apparatus; mitosis; endoplasmic reticulum; organelle inheritance; vesicle


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