Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control

doi:10.1083/jcb.200106141

Published 29 October 2001. doi:10.1083/jcb.200106141

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© The Rockefeller University Press, 0021-9525/2001/10/405 $5.00
The Journal of Cell Biology, Volume 155, Number 3, October 29, 2001 405-414

Article

Intraflagellar transport balances continuous turnover of outer doublet microtubules : implications for flagellar length control

Wallace F. Marshall and Joel L. Rosenbaum

Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520

Address correspondence to Wallace Marshall, KBT310 MCDB Dept., Yale University, New Haven, CT 06520-8103. Tel.: (203) 432-3473. Fax: (203) 432-6161. E-mail: wallace.marshall{at}yale.edu

A central question in cell biology is how cells determine thesize of their organelles. Flagellar length control is a convenientsystem for studying organelle size regulation. Mechanistic modelsproposed for flagellar length regulation have been constrainedby the assumption that flagella are static structures once theyare assembled. However, recent work has shown that flagellaare dynamic and are constantly turning over. We have determinedthat this turnover occurs at the flagellar tips, and that theassembly portion of the turnover is mediated by intraflagellartransport (IFT). Blocking IFT inhibits the incorporation oftubulin at the flagellar tips and causes the flagella to resorb.These results lead to a simple steady-state model for flagellarlength regulation by which a balance of assembly and disassemblycan effectively regulate flagellar length.

Key Words: flagella; tubulin; Chlamydomonas; kinesin; intraflagellar transport

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