Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text PDF (Full Text) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Short, B. Articles by Barr, F. A. Search for Related Content PubMed PubMed Citation Articles by Short, B. Articles by Barr, F. A. Social Bookmarking                      What's this?

¹ Department of Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried D-82152, Germany
² Division of Infection and Immunity, Faculty of Biomedical and Life Science, University of Glasgow, Glasgow, UK

Address correspondence to Francis Barr, Max-Planck-Institute of Biochemistry, Dept. of Cell Biology, Am Klopferspitz 18a, Martinsried D-85152, Germany. Tel.: 49-89-8578-3135. Fax: 49-89-8578-3102. E-mail: barr{at}biochem.mpg.de

Membrane traffic between the endoplasmic reticulum (ER) and Golgi apparatus and through the Golgi apparatus is a highly regulated process controlled by members of the rab GTPase family. The GTP form of rab1 regulates ER to Golgi transport by interaction with the vesicle tethering factor p115 and the cis-Golgi matrix protein GM130, also part of a complex with GRASP65 important for the organization of cis-Golgi cisternae. Here, we find that a novel coiled-coil protein golgin-45 interacts with the medial-Golgi matrix protein GRASP55 and the GTP form of rab2 but not other Golgi rab proteins. Depletion of golgin-45 disrupts the Golgi apparatus and causes a block in secretory protein transport. These results demonstrate that GRASP55 and golgin-45 form a rab2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure.

Key Words: Golgi apparatus; protein transport; rab GTPases; rab2; Golgi stacks

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Mountjoy, J. R., Xu, W., McLeod, D., Hyndman, D., Oko, R. (2008). RAB2A: A Major Subacrosomal Protein of Bovine Spermatozoa Implicated in Acrosomal Biogenesis. Biol. Reprod. 79: 223-232 [Abstract] [Full Text]  
• Struck, N. S., Herrmann, S., Langer, C., Krueger, A., Foth, B. J., Engelberg, K., Cabrera, A. L., Haase, S., Treeck, M., Marti, M., Cowman, A. F., Spielmann, T., Gilberger, T. W. (2008). Plasmodium falciparum possesses two GRASP proteins that are differentially targeted to the Golgi complex via a higher- and lower-eukaryote-like mechanism. J. Cell Sci. 121: 2123-2129 [Abstract] [Full Text]  
• Ramirez, I. B.-R., de Graffenried, C. L., Ebersberger, I., Yelinek, J., He, C. Y., Price, A., Warren, G. (2008). TbG63, a golgin involved in Golgi architecture in Trypanosoma brucei. J. Cell Sci. 121: 1538-1546 [Abstract] [Full Text]  
• Mangahas, P. M., Yu, X., Miller, K. G., Zhou, Z. (2008). The small GTPase Rab2 functions in the removal of apoptotic cells in Caenorhabditis elegans. J. Cell Biol. 180: 357-373 [Abstract] [Full Text]  
• Sun, Y., Shestakova, A., Hunt, L., Sehgal, S., Lupashin, V., Storrie, B. (2007). Rab6 Regulates Both ZW10/RINT-1 and Conserved Oligomeric Golgi Complex-dependent Golgi Trafficking and Homeostasis. Mol. Biol. Cell 18: 4129-4142 [Abstract] [Full Text]  
• Haas, A. K., Yoshimura, S.-i., Stephens, D. J., Preisinger, C., Fuchs, E., Barr, F. A. (2007). Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells. J. Cell Sci. 120: 2997-3010 [Abstract] [Full Text]  
• Reddy, J. V., Burguete, A. S., Sridevi, K., Ganley, I. G., Nottingham, R. M., Pfeffer, S. R. (2006). A Functional Role for the GCC185 Golgin in Mannose 6-Phosphate Receptor Recycling. Mol. Biol. Cell 17: 4353-4363 [Abstract] [Full Text]  
• Sztul, E., Lupashin, V. (2006). Role of tethering factors in secretory membrane traffic. Am. J. Physiol. Cell Physiol. 290: C11-C26 [Abstract] [Full Text]  
• Kondylis, V., Spoorendonk, K. M., Rabouille, C. (2005). dGRASP Localization and Function in the Early Exocytic Pathway in Drosophila S2 Cells. Mol. Biol. Cell 16: 4061-4072 [Abstract] [Full Text]  
• Hicks, S. W., Machamer, C. E. (2005). Isoform-specific Interaction of Golgin-160 with the Golgi-associated Protein PIST. J. Biol. Chem. 280: 28944-28951 [Abstract] [Full Text]  
• Beard, M., Satoh, A., Shorter, J., Warren, G. (2005). A Cryptic Rab1-binding Site in the p115 Tethering Protein. J. Biol. Chem. 280: 25840-25848 [Abstract] [Full Text]  
• Derby, M. C., van Vliet, C., Brown, D., Luke, M. R., Lu, L., Hong, W., Stow, J. L., Gleeson, P. A. (2004). Mammalian GRIP domain proteins differ in their membrane binding properties and are recruited to distinct domains of the TGN. J. Cell Sci. 117: 5865-5874 [Abstract] [Full Text]  
• Larocca, M. C., Shanks, R. A., Tian, L., Nelson, D. L., Stewart, D. M., Goldenring, J. R. (2004). AKAP350 Interaction with cdc42 Interacting Protein 4 at the Golgi Apparatus. Mol. Biol. Cell 15: 2771-2781 [Abstract] [Full Text]  
• Preisinger, C., Short, B., De Corte, V., Bruyneel, E., Haas, A., Kopajtich, R., Gettemans, J., Barr, F. A. (2004). YSK1 is activated by the Golgi matrix protein GM130 and plays a role in cell migration through its substrate 14-3-3{zeta}. J. Cell Biol. 164: 1009-1020 [Abstract] [Full Text]  
• Cha, H., Smith, B. L., Gallo, K., Machamer, C. E., Shapiro, P. (2004). Phosphorylation of golgin-160 by mixed lineage kinase 3. J. Cell Sci. 117: 751-760 [Abstract] [Full Text]  
• Yoshimura, S.-i., Yamamoto, A., Misumi, Y., Sohda, M., Barr, F. A., Fujii, G., Shakoori, A., Ohno, H., Mihara, K., Nakamura, N. (2004). Dynamics of Golgi Matrix Proteins after the Blockage of ER to Golgi Transport. J Biochem 135: 201-216 [Abstract] [Full Text]  
• Puri, S., Telfer, H., Velliste, M., Murphy, R. F., Linstedt, A. D. (2004). Dispersal of Golgi matrix proteins during mitotic Golgi disassembly. J. Cell Sci. 117: 451-456 [Abstract] [Full Text]  
• Puri, S., Linstedt, A. D. (2003). Capacity of the Golgi Apparatus for Biogenesis from the Endoplasmic Reticulum. Mol. Biol. Cell 14: 5011-5018 [Abstract] [Full Text]  
• Cerveny, K. L., Jensen, R. E. (2003). The WD-repeats of Net2p Interact with Dnm1p and Fis1p to Regulate Division of Mitochondria. Mol. Biol. Cell 14: 4126-4139 [Abstract] [Full Text]  
• Neef, R., Preisinger, C., Sutcliffe, J., Kopajtich, R., Nigg, E. A., Mayer, T. U., Barr, F. A. (2003). Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J. Cell Biol. 162: 863-876 [Abstract] [Full Text]  
• Nickas, M. E., Schwartz, C., Neiman, A. M. (2003). Ady4p and Spo74p Are Components of the Meiotic Spindle Pole Body That Promote Growth of the Prospore Membrane in Saccharomyces cerevisiae. Eukaryot Cell 2: 431-445 [Abstract] [Full Text]  
• Luke, M. R., Kjer-Nielsen, L., Brown, D. L., Stow, J. L., Gleeson, P. A. (2003). GRIP Domain-mediated Targeting of Two New Coiled-coil Proteins, GCC88 and GCC185, to Subcompartments of the trans-Golgi Network. J. Biol. Chem. 278: 4216-4226 [Abstract] [Full Text]  
• Diao, A., Rahman, D., Pappin, D. J.C., Lucocq, J., Lowe, M. (2003). The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation. J. Cell Biol. 160: 201-212 [Abstract] [Full Text]  
• Wang, T., Hong, W. (2002). Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus through Rab34 Interaction with Rab-interacting Lysosomal Protein. Mol. Biol. Cell 13: 4317-4332 [Abstract] [Full Text]  
• Tuschl, T., Borkhardt, A. (2002). Small Interfering RNAs: A Revolutionary Tool for the Analysis of Gene Function and Gene Therapy. Mol. Interv. 2: 158-167 [Abstract] [Full Text]  
• Shorter, J., Beard, M. B., Seemann, J., Dirac-Svejstrup, A. B., Warren, G. (2002). Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J. Cell Biol. 157: 45-62 [Abstract] [Full Text]  
• Pfeffer, S. R. (2001). Constructing a Golgi complex. J. Cell Biol. 155: 873-876 [Abstract] [Full Text]  
• Barr, F. A., Preisinger, C., Kopajtich, R., Korner, R. (2001). Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus. J. Cell Biol. 155: 885-892 [Abstract] [Full Text]  
• Shorter, J., Beard, M. B., Seemann, J., Dirac-Svejstrup, A. B., Warren, G. (2002). Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J. Cell Biol. 157: 45-62 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents