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Published 24 December 2001. doi:10.1083/jcb.200105109
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© The Rockefeller University Press, 0021-9525/2001/12/1129 $5.00
The Journal of Cell Biology, Volume 155, Number 7, December 24, 2001 1129-1136

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 The lipid phosphatase activity of PTEN is critical for stabilizing intercellular junctions and reverting invasiveness

Larissa Kotelevets1,2, Jolanda van Hengel2, Erik Bruyneel3, Marc Mareel3, Frans van Roy2 and Eric Chastre1,2

1 Institut National de la Santé et de la Recherche Médicale (INSERM) U410, Faculté de Médecine Bichat, 75018 Paris, France
2 Molecular Cell Biology Unit, Department of Molecular Biology, VIB-Ghent University, B-9000 Ghent, Belgium
3 Laboratory of Experimental Cancerology, Ghent University Hospital, B-9000 Ghent, Belgium

Address correspondence to Eric Chastre, INSERM U410, Faculté de Médecine X. Bichat, 16 rue Huchard, 75018 Paris, France. Tel.: 33-14-485-6139. Fax: 33-14-228-8765. E-mail: chastre{at}bichat.inserm.fr

To analyze the implication of PTEN in the control of tumor cell invasiveness, the canine kidney epithelial cell lines MDCKras-f and MDCKts-src, expressing activated Ras and a temperature-sensitive v-Src tyrosine kinase, respectively, were transfected with PTEN expression vectors. Likewise, the human PTEN-defective glioblastoma cell lines U87MG and U373MG, the melanoma cell line FM-45, and the prostate carcinoma cell line PC-3 were transfected. We demonstrate that ectopic expression of wild-type PTEN in MDCKts-src cells, but not expression of PTEN mutants deficient in either the lipid or both the lipid and protein phosphatase activities, reverted the morphological transformation, induced cell–cell aggregation, and suppressed the invasive phenotype in an E-cadherin–dependent manner. In contrast, overexpression of wild-type PTEN did not counteract Ras-induced invasiveness of MDCKras-f cells expressing low levels of E-cadherin. PTEN effects were not associated with marked changes in accumulation or phosphorylation levels of E-cadherin and associated catenins. Wild-type, but not mutant, PTEN also reverted the invasive phenotype of U87MG, U373MG, PC-3, and FM-45 cells. Interestingly, PTEN effects were mimicked by N-cadherin–neutralizing antibody in the glioblastoma cell lines. Our data confirm the differential activities of E- and N-cadherin on invasiveness and suggest that the lipid phosphatase activity of PTEN exerts a critical role in stabilizing junctional complexes and restraining invasiveness.

Key Words: PTEN; invasiveness; E-cadherin; Src; PI3 kinase


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