CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis

doi:10.1083/jcb.200108125

Published 24 December 2001. doi:10.1083/jcb.200108125

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© The Rockefeller University Press, 0021-9525/2001/12/1147 $5.00
The Journal of Cell Biology, Volume 155, Number 7, December 24, 2001 1147-1158

Article

CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis

Samantha G. Zeitlin, Richard D. Shelby and Kevin F. Sullivan

Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037

Address correspondence to Kevin F. Sullivan, The Scripps Research Institute, maildrop MB-38, 10550 North Torrey Pines Rd., La Jolla, CA 92037. Tel.: (858) 784-2350. Fax: (858) 784-9927. E-mail: sullivan{at}scripps.edu

Aurora B is a mitotic protein kinase that phosphorylates histoneH3, behaves as a chromosomal passenger protein, and functionsin cytokinesis. We investigated a role for Aurora B with respectto human centromere protein A (CENP-A), a centromeric histoneH3 homologue. Aurora B concentrates at centromeres in earlyG2, associates with histone H3 and centromeres at the timeswhen histone H3 and CENP-A are phosphorylated, and phosphorylateshistone H3 and CENP-A in vitro at a similar target serine residue.Dominant negative phosphorylation site mutants of CENP-A resultin a delay at the terminal stage of cytokinesis (cell separation).The only molecular defects detected in analysis of 22 chromosomal,spindle, and regulatory proteins were disruptions in localizationof inner centromere protein (INCENP), Aurora B, and a putativepartner phosphatase, PP1 ${gamma}$ 1. Our data support a model where CENP-Aphosphorylation is involved in regulating Aurora B, INCENP,and PP1 ${gamma}$ 1 targeting within the cell. These experiments identifyan unexpected role for the kinetochore in regulation of cytokinesis.

Key Words: CENP-A; Aurora B; midbody; PP1 ${gamma}$ 1; cytokinesis

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