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Published 7 January 2002. doi:10.1083/jcb.200110059
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© The Rockefeller University Press, 0021-9525/2002/1/75 $5.00
The Journal of Cell Biology, Volume 156, Number 1, January 7, 2002 75-86

Article

 Induction of maturation-promoting factor during Xenopus oocyte maturation uncouples Ca2+ store depletion from store-operated Ca2+ entry

Khaled Machaca and Shirley Haun

Address correspondence to Khaled Machaca, Ph.D., Department of Physiology, Slot 505, 4301 West Markham St., University of Arkansas Medical Science, Little Rock, AR 72205. Tel.: (501) 603-1596. Fax: (501) 686-8167. E-mail: machacakhaleda{at}uams.edu

Department of Physiology and Biophysics, University of Arkansas Medical Science, Little Rock, AR 72205

During oocyte maturation, eggs acquire the ability to generate specialized Ca2+ signals in response to sperm entry. Such Ca2+ signals are crucial for egg activation and the initiation of embryonic development. We examined the regulation during Xenopus oocyte maturation of store-operated Ca2+ entry (SOCE), an important Ca2+ influx pathway in oocytes and other nonexcitable cells. We have previously shown that SOCE inactivates during Xenopus oocyte meiosis. SOCE inactivation may be important in preventing premature egg activation. In this study, we investigated the correlation between SOCE inactivation and the Mos–mitogen-activated protein kinase (MAPK)–maturation-promoting factor (MPF) kinase cascade, which drives Xenopus oocyte maturation. SOCE inactivation at germinal vesicle breakdown coincides with an increase in the levels of MAPK and MPF. By differentially inducing Mos, MAPK, and MPF, we demonstrate that the activation of MPF is necessary for SOCE inactivation during oocyte maturation. In contrast, sustained high levels of Mos kinase and the MAPK cascade have no effect on SOCE activation. We further show that preactivated SOCE is not inactivated by MPF, suggesting that MPF does not block Ca2+ influx through SOCE channels, but rather inhibits coupling between store depletion and SOCE activation.

Key Words: calcium signaling; store-operated Ca2+ entry; oocyte maturation; Xenopus laevis; maturation-promoting factor


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