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Published 21 January 2002. doi:10.1083/jcb.200107135
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© The Rockefeller University Press, 0021-9525/2002/1/241 $5.00
The Journal of Cell Biology, Volume 156, Number 2, January 21, 2002 241-248

Report

 The conserved Pkh–Ypk kinase cascade is required for endocytosis in yeast

Amy K.A. deHart, Joshua D. Schnell, Damian A. Allen and Linda Hicke

Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL, 60208

Address correspondence to Linda Hicke, Dept. of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208. Tel.: (847) 467-4490. Fax: (847) 467-1380. E-mail: l-hicke{at}northwestern.edu

Internalization of activated signaling receptors by endocytosis is one way cells downregulate extracellular signals. Like many signaling receptors, the yeast {alpha}-factor pheromone receptor is downregulated by hyperphosphorylation, ubiquitination, and subsequent internalization and degradation in the lysosome-like vacuole. In a screen to detect proteins involved in ubiquitin-dependent receptor internalization, we identified the sphingoid base–regulated serine–threonine kinase Ypk1. Ypk1 is a homologue of the mammalian serum– and glucocorticoid-induced kinase, SGK, which can substitute for Ypk1 function in yeast. The kinase activity of Ypk1 is required for receptor endocytosis because mutations in two residues important for its catalytic activity cause a severe defect in {alpha}-factor internalization. Ypk1 is required for both receptor-mediated and fluid-phase endocytosis, and is not necessary for receptor phosphorylation or ubiquitination. Ypk1 itself is phosphorylated by Pkh kinases, homologues of mammalian PDK1. The threonine in Ypk1 that is phosphorylated by Pkh1 is required for efficient endocytosis, and pkh mutant cells are defective in {alpha}-factor internalization and fluid-phase endocytosis. These observations demonstrate that Ypk1 acts downstream of the Pkh kinases to control endocytosis by phosphorylating components of the endocytic machinery.

Key Words: serine–threonine kinase; sphingolipid; receptor downregulation; endocytosis; internalization


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