Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis

doi:10.1083/jcb.200109059

Published 29 April 2002. doi:10.1083/jcb.200109059

This Article

	Full Text
	PDF (Full Text)
	PPT slides of all figures
	Supplemental Material Index
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Niethammer, P.
	Articles by Schachner, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Niethammer, P.
	Articles by Schachner, M.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2002/4/521 $5.00
The Journal of Cell Biology, Volume 157, Number 3, April 29, 2002 521-532

Article

Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis

Philipp Niethammer¹, Markus Delling¹, Vladimir Sytnyk¹, Alexander Dityatev¹, Kiyoko Fukami² and Melitta Schachner¹

¹ Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
² Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato-ku, 108-8639 Tokyo, Japan

Address correspondence to Melitta Schachner, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany. Tel.: 49-40-42803-6246. Fax: 49-40-42803-6248. E-mail: melitta.schachner{at}zmnh.uni-hamburg.de

The neural cell adhesion molecule (NCAM) has been reported tostimulate neuritogenesis either via nonreceptor tyrosine kinasesor fibroblast growth factor (FGF) receptor. Here we show thatlipid raft association of NCAM is crucial for activation ofthe nonreceptor tyrosine kinase pathway and induction of neuriteoutgrowth. Transfection of hippocampal neurons of NCAM-deficientmice revealed that of the three major NCAM isoforms only NCAM140can act as a homophilic receptor that induces neurite outgrowth.Disruption of NCAM140 raft association either by mutation ofNCAM140 palmitoylation sites or by lipid raft destruction attenuatesactivation of the tyrosine focal adhesion kinase and extracellularsignal–regulated kinase 1/2, completely blocking neuriteoutgrowth. Likewise, NCAM-triggered neurite outgrowth is alsocompletely blocked by a specific FGF receptor inhibitor, indicatingthat cosignaling via raft-associated kinases and FGF receptoris essential for neuritogenesis.

Key Words: neurite outgrowth; NCAM; lipid rafts; palmitoylation; FGF receptor

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Bodrikov, V., Sytnyk, V., Leshchyns'ka, I., den Hertog, J., Schachner, M. (2008). NCAM induces CaMKII{alpha}-mediated RPTP{alpha} phosphorylation to enhance its catalytic activity and neurite outgrowth. J. Cell Biol. 182: 1185-1200 [Abstract] [Full Text]
Ponimaskin, E., Dityateva, G., Ruonala, M. O., Fukata, M., Fukata, Y., Kobe, F., Wouters, F. S., Delling, M., Bredt, D. S., Schachner, M., Dityatev, A. (2008). Fibroblast Growth Factor-Regulated Palmitoylation of the Neural Cell Adhesion Molecule Determines Neuronal Morphogenesis. J. Neurosci. 28: 8897-8907 [Abstract] [Full Text]
Brackenbury, W. J., Davis, T. H., Chen, C., Slat, E. A., Detrow, M. J., Dickendesher, T. L., Ranscht, B., Isom, L. L. (2008). Voltage-Gated Na+ Channel {beta}1 Subunit-Mediated Neurite Outgrowth Requires Fyn Kinase and Contributes to Postnatal CNS Development In Vivo. J. Neurosci. 28: 3246-3256 [Abstract] [Full Text]
Roduit, C., van der Goot, F. G., De Los Rios, P., Yersin, A., Steiner, P., Dietler, G., Catsicas, S., Lafont, F., Kasas, S. (2008). Elastic Membrane Heterogeneity of Living Cells Revealed by Stiff Nanoscale Membrane Domains. Biophys. J 94: 1521-1532 [Abstract] [Full Text]
Diestel, S., Schaefer, D., Cremer, H., Schmitz, B. (2007). NCAM is ubiquitylated, endocytosed and recycled in neurons. J. Cell Sci. 120: 4035-4049 [Abstract] [Full Text]
Conchonaud, F., Nicolas, S., Amoureux, M.-C., Menager, C., Marguet, D., Lenne, P.-F., Rougon, G., Matarazzo, V. (2007). Polysialylation Increases Lateral Diffusion of Neural Cell Adhesion Molecule in the Cell Membrane. J. Biol. Chem. 282: 26266-26274 [Abstract] [Full Text]
Buff, H., Smith, A. C., Korey, C. A. (2007). Genetic Modifiers of Drosophila Palmitoyl-Protein Thioesterase 1-Induced Degeneration. Genetics 176: 209-220 [Abstract] [Full Text]
Wang, X., Hisha, H., Cui, W., Song, C., Mizokami, T., Okazaki, S., Li, Q., Feng, W., Kato, J., Jiang, S., Fan, H., Ikehara, S. (2007). The characteristics of hematopoietic stem cells from autoimmune-prone mice and the role of neural cell adhesion molecules in abnormal proliferation of these cells in MRL/lpr mice. haematol 92: 300-307 [Abstract] [Full Text]
Brown, D. A. (2006). Lipid Rafts, Detergent-Resistant Membranes, and Raft Targeting Signals.. Physiology 21: 430-439 [Abstract] [Full Text]
Sanchez-Heras, E., Howell, F. V., Williams, G., Doherty, P. (2006). The Fibroblast Growth Factor Receptor Acid Box Is Essential for Interactions with N-Cadherin and All of the Major Isoforms of Neural Cell Adhesion Molecule. J. Biol. Chem. 281: 35208-35216 [Abstract] [Full Text]
Senkov, O., Sun, M., Weinhold, B., Gerardy-Schahn, R., Schachner, M., Dityatev, A. (2006). Polysialylated Neural Cell Adhesion Molecule Is Involved in Induction of Long-Term Potentiation and Memory Acquisition and Consolidation in a Fear-Conditioning Paradigm.. J. Neurosci. 26: 10888-109898 [Abstract] [Full Text]
Wang, X., Hisha, H., Taketani, S., Inaba, M., Li, Q., Cui, W., Song, C., Fan, T., Cui, Y., Guo, K., Yang, G., Fan, H., Lian, Z., Gershwin, M. E., Ikehara, S. (2005). Neural Cell Adhesion Molecule Contributes to Hemopoiesis-Supporting Capacity of Stromal Cell Lines. Stem Cells 23: 1389-1399 [Abstract] [Full Text]
Shogomori, H., Hammond, A. T., Ostermeyer-Fay, A. G., Barr, D. J., Feigenson, G. W., London, E., Brown, D. A. (2005). Palmitoylation and Intracellular Domain Interactions Both Contribute to Raft Targeting of Linker for Activation of T Cells. J. Biol. Chem. 280: 18931-18942 [Abstract] [Full Text]
Causeret, M., Taulet, N., Comunale, F., Favard, C., Gauthier-Rouviere, C. (2005). N-Cadherin Association with Lipid Rafts Regulates Its Dynamic Assembly at Cell-Cell Junctions in C2C12 Myoblasts. Mol. Biol. Cell 16: 2168-2180 [Abstract] [Full Text]
Santuccione, A., Sytnyk, V., Leshchyns'ka, I., Schachner, M. (2005). Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59fyn and to enhance neurite outgrowth. J. Cell Biol. 169: 341-354 [Abstract] [Full Text]
Herincs, Z., Corset, V., Cahuzac, N., Furne, C., Castellani, V., Hueber, A.-O., Mehlen, P. (2005). DCC association with lipid rafts is required for netrin-1-mediated axon guidance. J. Cell Sci. 118: 1687-1692 [Abstract] [Full Text]
Bodrikov, V., Leshchyns'ka, I., Sytnyk, V., Overvoorde, J., den Hertog, J., Schachner, M. (2005). RPTP{alpha} is essential for NCAM-mediated p59fyn activation and neurite elongation. J. Cell Biol. 168: 127-139 [Abstract] [Full Text]
Davis, T. H., Chen, C., Isom, L. L. (2004). Sodium Channel {beta}1 Subunits Promote Neurite Outgrowth in Cerebellar Granule Neurons. J. Biol. Chem. 279: 51424-51432 [Abstract] [Full Text]
Crnic, I., Strittmatter, K., Cavallaro, U., Kopfstein, L., Jussila, L., Alitalo, K., Christofori, G. (2004). Loss of Neural Cell Adhesion Molecule Induces Tumor Metastasis by Up-regulating Lymphangiogenesis. Cancer Res. 64: 8630-8638 [Abstract] [Full Text]
Washbourne, P., Dityatev, A., Scheiffele, P., Biederer, T., Weiner, J. A., Christopherson, K. S., El-Husseini, A. (2004). Cell Adhesion Molecules in Synapse Formation. J. Neurosci. 24: 9244-9249 [Abstract] [Full Text]
Dityatev, A., Dityateva, G., Sytnyk, V., Delling, M., Toni, N., Nikonenko, I., Muller, D., Schachner, M. (2004). Polysialylated Neural Cell Adhesion Molecule Promotes Remodeling and Formation of Hippocampal Synapses. J. Neurosci. 24: 9372-9382 [Abstract] [Full Text]
Falk, J., Thoumine, O., Dequidt, C., Choquet, D., Faivre-Sarrailh, C. (2004). NrCAM Coupling to the Cytoskeleton Depends on Multiple Protein Domains and Partitioning into Lipid Rafts. Mol. Biol. Cell 15: 4695-4709 [Abstract] [Full Text]
Butchbach, M. E. R., Tian, G., Guo, H., Lin, C.-l. G. (2004). Association of Excitatory Amino Acid Transporters, Especially EAAT2, with Cholesterol-rich Lipid Raft Microdomains: IMPORTANCE FOR EXCITATORY AMINO ACID TRANSPORTER LOCALIZATION AND FUNCTION. J. Biol. Chem. 279: 34388-34396 [Abstract] [Full Text]
Cambon, K., Hansen, S. M., Venero, C., Herrero, A. I., Skibo, G., Berezin, V., Bock, E., Sandi, C. (2004). A Synthetic Neural Cell Adhesion Molecule Mimetic Peptide Promotes Synaptogenesis, Enhances Presynaptic Function, and Facilitates Memory Consolidation. J. Neurosci. 24: 4197-4204 [Abstract] [Full Text]
Decker, L., ffrench-Constant, C. (2004). Lipid Rafts and Integrin Activation Regulate Oligodendrocyte Survival. J. Neurosci. 24: 3816-3825 [Abstract] [Full Text]
Islam, R., Kristiansen, L. V., Romani, S., Garcia-Alonso, L., Hortsch, M. (2004). Activation of EGF Receptor Kinase by L1-mediated Homophilic Cell Interactions. Mol. Biol. Cell 15: 2003-2012 [Abstract] [Full Text]
Thorp, E. B., Gallagher, T. M. (2004). Requirements for CEACAMs and Cholesterol during Murine Coronavirus Cell Entry. J. Virol. 78: 2682-2692 [Abstract] [Full Text]
Shin, D. M., Korada, S., Raballo, R., Shashikant, C. S., Simeone, A., Taylor, J. R., Vaccarino, F. (2004). Loss of Glutamatergic Pyramidal Neurons in Frontal and Temporal Cortex Resulting from Attenuation of FGFR1 Signaling Is Associated with Spontaneous Hyperactivity in Mice. J. Neurosci. 24: 2247-2258 [Abstract] [Full Text]
Bukalo, O., Fentrop, N., Lee, A. Y. W., Salmen, B., Law, J. W. S., Wotjak, C. T., Schweizer, M., Dityatev, A., Schachner, M. (2004). Conditional Ablation of the Neural Cell Adhesion Molecule Reduces Precision of Spatial Learning, Long-Term Potentiation, and Depression in the CA1 Subfield of Mouse Hippocampus. J. Neurosci. 24: 1565-1577 [Abstract] [Full Text]
Papoucheva, E., Dumuis, A., Sebben, M., Richter, D. W., Ponimaskin, E. G. (2004). The 5-Hydroxytryptamine(1A) Receptor Is Stably Palmitoylated, and Acylation Is Critical for Communication of Receptor with Gi Protein. J. Biol. Chem. 279: 3280-3291 [Abstract] [Full Text]
Seidenfaden, R., Krauter, A., Schertzinger, F., Gerardy-Schahn, R., Hildebrandt, H. (2003). Polysialic Acid Directs Tumor Cell Growth by Controlling Heterophilic Neural Cell Adhesion Molecule Interactions. Mol. Cell. Biol. 23: 5908-5918 [Abstract] [Full Text]
Peng, H., Derrick, B. E., Martinez, J. L. Jr (2003). Identification of Upregulated SCG10 mRNA Expression Associated with Late-Phase Long-Term Potentiation in the Rat Hippocampal Schaffer-CA1 Pathway In Vivo. J. Neurosci. 23: 6617-6626 [Abstract] [Full Text]
Leshchyns'ka, I., Sytnyk, V., Morrow, J. S., Schachner, M. (2003). Neural cell adhesion molecule (NCAM) association with PKC{beta}2 via {beta}I spectrin is implicated in NCAM-mediated neurite outgrowth. J. Cell Biol. 161: 625-639 [Abstract] [Full Text]
Stockklausner, C., Klocker, N. (2003). Surface Expression of Inward Rectifier Potassium Channels Is Controlled by Selective Golgi Export. J. Biol. Chem. 278: 17000-17005 [Abstract] [Full Text]
Nakai, Y., Kamiguchi, H. (2002). Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner. J. Cell Biol. 159: 1097-1108 [Abstract] [Full Text]
Delling, M., Wischmeyer, E., Dityatev, A., Sytnyk, V., Veh, R. W., Karschin, A., Schachner, M. (2002). The Neural Cell Adhesion Molecule Regulates Cell-Surface Delivery of G-Protein-Activated Inwardly Rectifying Potassium Channels Via Lipid Rafts. J. Neurosci. 22: 7154-7164 [Abstract] [Full Text]