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Published online 20 May 2002. doi:10.1083/jcb.200108150
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© The Rockefeller University Press, 0021-9525/2002/5/851 $5.00
The Journal of Cell Biology, Volume 157, Number 5, May 28, 2002 851-864

Article

 Identification of a novel population of muscle stem cells in mice : potential for muscle regeneration



Zhuqing Qu-Petersen1, Bridget Deasy1, Ron Jankowski1, Makato Ikezawa1, James Cummins1, Ryan Pruchnic1, John Mytinger1, Baohong Cao1, Charley Gates1, Anton Wernig2 and Johnny Huard1

1 Growth and Development Laboratory, Children's Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15260
2 Departments of Physiology and Neurophysiology, University of Bonn, D-53111 Bonn, Germany

Address correspondence to J. Huard, Growth and Development Laboratory, Children's Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, 3705 5th Ave., Pittsburgh, PA 15213-2582. Tel.: (412) 692-7807. Fax: (412) 692-7095. E-mail: jhuard+{at}pitt.edu

Three populations of myogenic cells were isolated from normal mouse skeletal muscle based on their adhesion characteristics and proliferation behaviors. Although two of these populations displayed satellite cell characteristics, a third population of long-time proliferating cells expressing hematopoietic stem cell markers was also identified. This third population comprises cells that retain their phenotype for more than 30 passages with normal karyotype and can differentiate into muscle, neural, and endothelial lineages both in vitro and in vivo. In contrast to the other two populations of myogenic cells, the transplantation of the long-time proliferating cells improved the efficiency of muscle regeneration and dystrophin delivery to dystrophic muscle. The long-time proliferating cells' ability to proliferate in vivo for an extended period of time, combined with their strong capacity for self-renewal, their multipotent differentiation, and their immune-privileged behavior, reveals, at least in part, the basis for the improvement of cell transplantation. Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell–mediated therapies.

Key Words: muscle-derived stem cells (MDSC); satellite cells; cell transplantation; dystrophin; mdx mice


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