JCB logo
BioLegend: Antibody Reagents
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 10 June 2002. doi:10.1083/jcb.200111012
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Façanha, A. L. O.
Right arrow Articles by Ekwall, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Façanha, A. L. O.
Right arrow Articles by Ekwall, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2002/6/1029 $5.00
The Journal of Cell Biology, Volume 157, Number 6, June 10, 2002 1029-1040

Article

 The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics

Anna L. Okorokova Façanha1, Henrik Appelgren2, Mohammad Tabish2, Lev Okorokov1 and Karl Ekwall2

1 Laboratório Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Horto, CEP 28015-620, Brazil
2 Karolinska Institutet, Department of Biosciences Novum/University College Sodertorn, Deptartment of Natural Sciences, S-141 04 Huddinge, Sweden

Address correspondence to Karl Ekwall, University College Sodertorn, Dept. of Natural Sciences, Alfred Nobels Alle 3, S-141 52 Huddinge, Sweden. Tel.: 46-8-608-4713. Fax: 46-8-608-4510. E-mail: karl.ekwall{at}cbt.ki.se

Here we describe the phenotypic characterization of the cta4+ gene, encoding a novel member of the P4 family of P-type ATPases of fission yeast. The cta4{Delta} mutant is temperature sensitive and cold sensitive lethal and displays several morphological defects in cell polarity and cytokinesis. Microtubules are generally destabilized in cells lacking Cta4p. The microtubule length is decreased, and the number of microtubules per cell is increased. This is concomitant with an increase in the number of microtubule catastrophe events in the midzone of the cell. These defects are likely due to a general imbalance in cation homeostasis. Immunofluorescence microscopy and membrane fractionation experiments revealed that green fluorescent protein–tagged Cta4 localizes to the ER. Fluorescence resonance energy transfer experiments in living cells using the yellow cameleon indicator for Ca2+ indicated that Cta4p regulates the cellular Ca2+ concentration. Thus, our results reveal a link between cation homeostasis and the control of cell shape, microtubule dynamics, and cytokinesis, and appoint Ca2+ as a key ion in controlling these processes.

Key Words: Schizosaccharomyces pombe; P-type ATPase; endoplasmic reticulum; calcium; microtubule


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents