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Shwetal Mehta¹, Xian Mei Yang¹, Clarence S. Chan¹, Melanie J. Dobson², Makkuni Jayaram¹ and Soundarapandian Velmurugan¹

¹ Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712
² Department of Biochemistry and Molecular Biology, Dalhousie University, Nova Scotia, Canada B3H 4H7

Address correspondence to Soundarapandian Velmurugan, Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712. Tel.: (512) 471-5537. Fax: (512) 471-5546. E-mail: velmurugan{at}mail.utexas.edu

The yeast 2 micron plasmid achieves high fidelity segregation by coupling its partitioning pathway to that of the chromosomes. Mutations affecting distinct steps of chromosome segregation cause the plasmid to missegregate in tandem with the chromosomes. In the absence of the plasmid stability system, consisting of the Rep1 and Rep2 proteins and the STB DNA, plasmid and chromosome segregations are uncoupled. The Rep proteins, acting in concert, recruit the yeast cohesin complex to the STB locus. The periodicity of cohesin association and dissociation is nearly identical for the plasmid and the chromosomes. The timely disassembly of cohesin is a prerequisite for plasmid segregation. Cohesin-mediated pairing and unpairing likely provides a counting mechanism for evenly partitioning plasmids either in association with or independently of the chromosomes.

Key Words: yeast plasmid segregation; chromatin immunoprecipitation; monohybrid assay; cohesin recruitment; nondegradable Mcd1p

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