Assembly of centrosomal proteins and microtubule organization depends on PCM-1

doi:10.1083/jcb.200204023

Published 28 October 2002. doi:10.1083/jcb.200204023

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© The Rockefeller University Press, 0021-9525/2002/10/255 $5.00
The Journal of Cell Biology, Volume 159, Number 2, 255-266

Article

Assembly of centrosomal proteins and microtubule organization depends on PCM-1

Alexander Dammermann and Andreas Merdes

Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, UK

Address correspondence to Andreas Merdes, Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Rd., Edinburgh EH9 3JR, UK. Tel.: 44-131-650-7075. Fax: 44-131-650-7360. E-mail: a.merdes{at}ed.ac.uk

The protein PCM-1 localizes to cytoplasmic granules known as"centriolar satellites" that are partly enriched around thecentrosome. We inhibited PCM-1 function using a variety of approaches:microinjection of antibodies into cultured cells, overexpressionof a PCM-1 deletion mutant, and specific depletion of PCM-1by siRNA. All approaches led to reduced targeting of centrin,pericentrin, and ninein to the centrosome. Similar effects wereseen upon inhibition of dynactin by dynamitin, and after prolongedtreatment of cells with the microtubule inhibitor nocodazole.Inhibition or depletion of PCM-1 function further disruptedthe radial organization of microtubules without affecting microtubulenucleation. Loss of microtubule organization was also observedafter centrin or ninein depletion. Our data suggest that PCM-1–containingcentriolar satellites are involved in the microtubule- and dynactin-dependentrecruitment of proteins to the centrosome, of which centrinand ninein are required for interphase microtubule organization.

Key Words: centrosome; microtubules; pericentriolar material; RNAi; dynein

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