The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation

doi:10.1083/jcb.200207045

Published 21 January 2003. doi:10.1083/jcb.200207045

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© The Rockefeller University Press, 0021-9525/2003/1/201 $5.00
The Journal of Cell Biology, Volume 160, Number 2, 201-212

Article

The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation

Aipo Diao¹, Dinah Rahman³, Darryl J.C. Pappin³, John Lucocq² and Martin Lowe¹

¹ School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
² School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
³ Proteomics Section, Medical Research Council Clinical Sciences Centre, Imperial College of Science, Technology, and Medicine, Hammersmith Hospital, London W12 0NN, UK

Address correspondence to Martin Lowe, School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK. Tel.: 44-161-275-5387. Fax: 44-161-275-5082. E-mail: lowe{at}man.ac.uk

Fragmentation of the mammalian Golgi apparatus during mitosisrequires the phosphorylation of a specific subset of Golgi-associatedproteins. We have used a biochemical approach to characterizethese proteins and report here the identification of golgin-84as a novel mitotic target. Using cryoelectron microscopy wecould localize golgin-84 to the cis-Golgi network and foundthat it is enriched on tubules emanating from the lateral edgesof, and often connecting, Golgi stacks. Golgin-84 binds to activerab1 but not cis-Golgi matrix proteins. Overexpression or depletionof golgin-84 results in fragmentation of the Golgi ribbon. Strikingly,the Golgi ribbon is converted into mini-stacks constitutingonly $~$ 25% of the volume of a normal Golgi apparatus upon golgin-84depletion. These mini-stacks are able to carry out protein transport,though with reduced efficiency compared with a normal Golgiapparatus. Our results suggest that golgin-84 plays a key rolein the assembly and maintenance of the Golgi ribbon in mammaliancells.

Key Words: Golgi apparatus; golgin; mitosis; Golgi structure; protein phosphorylation

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