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Published 3 March 2003. doi:10.1083/jcb.200212080
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© The Rockefeller University Press, 0021-9525/2003/3/657 $5.00
The Journal of Cell Biology, Volume 160, Number 5, 657-670

Article

 Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1ß and SMC3

Maureen Eijpe1, Hildo Offenberg1, Rolf Jessberger2, Ekaterina Revenkova2 and Christa Heyting1

1 Molecular Genetics Group, Wageningen University, 6703 BD Wageningen, Netherlands
2 Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mt. Sinai School of Medicine, New York, NY 10029

Address correspondence to Christa Heyting, Molecular Genetics Group, Botanical Center, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands. Tel.: 31-317-482150. Fax: 31-317-483146. E-mail: christa.heyting{at}wur.nl

In meiotic prophase, the sister chromatids of each chromosome develop a common axial element (AE) that is integrated into the synaptonemal complex (SC). We analyzed the incorporation of sister chromatid cohesion proteins (cohesins) and other AE components into AEs. Meiotic cohesin REC8 appeared shortly before premeiotic S phase in the nucleus and formed AE-like structures (REC8-AEs) from premeiotic S phase on. Subsequently, meiotic cohesin SMC1ß, cohesin SMC3, and AE proteins SCP2 and SCP3 formed dots along REC8-AEs, which extended and fused until they lined REC8-AEs along their length. In metaphase I, SMC1ß, SMC3, SCP2, and SCP3 disappeared from the chromosome arms and accumulated around the centromeres, where they stayed until anaphase II. In striking contrast, REC8 persisted along the chromosome arms until anaphase I and near the centromeres until anaphase II. We propose that REC8 provides a basis for AE formation and that the first steps in AE assembly do not require SMC1ß, SMC3, SCP2, and SCP3. Furthermore, SMC1ß, SMC3, SCP2, and SCP3 cannot provide arm cohesion during metaphase I. We propose that REC8 then provides cohesion. RAD51 and/or DMC1 coimmunoprecipitates with REC8, suggesting that REC8 may also provide a basis for assembly of recombination complexes.

Key Words: meiosis; axial element; rat; sister chromatid cohesion; synaptonemal complex


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