JCB logo
MBoC5 from Garland Science
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 7 July 2003. doi:10.1083/jcb.200303019
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rupp, G.
Right arrow Articles by Porter, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rupp, G.
Right arrow Articles by Porter, M. E.
Right arrowPubmed/NCBI databases
*Nucleotide*Protein
*UniGene
*Substance via MeSH
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2003/7/47 $5.00
The Journal of Cell Biology, Volume 162, Number 1, 47-57

Article

 A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest–specific gene product

Gerald Rupp1,2 and Mary E. Porter1

1 Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, MN 55455
2 Department of Anatomy, Southern Illinois University School of Medicine, Carbondale, IL 62901

Address correspondence to Mary E. Porter, Department of Genetics, Cell Biology, and Development, 6-160 Jackson Hall, 321 Church St., SE, Minneapolis, MN 55455. Tel.: (612) 626-1901. Fax: (612) 625-4648. E-mail: mary-p{at}biosci.cbs.umn.edu

The dynein regulatory complex (DRC) is an important intermediate in the pathway that regulates flagellar motility. To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant obtained by insertional mutagenesis. The pf2-4 mutant displays an altered waveform that results in slow swimming cells. EM analysis reveals defects in DRC structure that can be rescued by reintroduction of the wild-type PF2 gene. Immunolocalization studies show that the PF2 protein is distributed along the length of the axoneme, where it is part of a discrete complex of polypeptides. PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest–specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin. PF2 and its homologues appear to be universal components of motile axonemes that are required for DRC assembly and the regulation of flagellar motility. The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.

Key Words: dynein; flagella; Gas8; Gas11; TLTF

* Abbreviations used in this paper: CP, central pair; DRC, dynein regulatory complex; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; RS, radial spoke; TLTF, T lymphocyte triggering factor.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Controlling flagellar strokes
Alan W. Dove
J. Cell Biol. 2003 162: 11. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents