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Published online 25 August 2003. doi:10.1083/jcb.200305048
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© The Rockefeller University Press, 0021-9525/2003/9/757 $5.00
The Journal of Cell Biology, Volume 162, Number 5, 757-764

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 Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells

Yasuhiko Terada, Yumi Uetake and Ryoko Kuriyama

Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455

Address correspondence to Ryoko Kuriyama, Dept. of Genetics, Cell Biology, and Development, 6-160 Jackson Hall, 321 Church St. SE, University of Minnesota, Minneapolis, MN 55455. Tel.: (612) 624-0471. Fax: (612) 626-6140. email: ryoko{at}lenti.med.umn.edu; or Yasuhiko Terada, Dept. of Genetics, Cell Biology, and Development, 6-160 Jackson Hall, 321 Church St. SE, University of Minnesota, Minneapolis, MN 55455. Tel.: (612) 626-4089. Fax: (612) 626-6140. email: terad002{at}umn.edu

A mitosis-specific Aurora-A kinase has been implicated in microtubule organization and spindle assembly in diverse organisms. However, exactly how Aurora-A controls the microtubule nucleation onto centrosomes is unknown. Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles. Aurora-A and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of {gamma}-tubulin and other centrosomal components to the centrosome. The NH2-terminal half of CNN interacts with {gamma}-tubulin, and induces cytoplasmic foci that can initiate microtubule nucleation in vivo and in vitro in both Drosophila and mammalian cells. These results suggest that Aurora-A regulates centrosome assembly by controlling the CNN's ability to targeting and/or anchoring {gamma}-tubulin to the centrosome and organizing microtubule-nucleating sites via its interaction with the COOH-terminal sequence of CNN.

Key Words: centrosomes; {gamma}-tubulin; microtubule nucleation; mitotic spindle; MTOC

The online version of this article includes supplemental material.

Abbreviations used in this paper: CNN, centrosomin; {gamma}-TuRC, {gamma}-tubulin–containing ring complex; MTOC, microtubule-organizing center; RNAi, RNA interference.


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