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Published online 25 August 2003. doi:10.1083/jcb.200306009
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© The Rockefeller University Press, 0021-9525/2003/9/863 $5.00
The Journal of Cell Biology, Volume 162, Number 5, 863-876

Article

 Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis

Rüdiger Neef1, Christian Preisinger1, Josephine Sutcliffe1, Robert Kopajtich1, Erich A. Nigg2, Thomas U. Mayer2 and Francis A. Barr1,2

1 Intracellular Protein Transport, Independent Junior Research Group
2 Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany

Address correspondence to Francis Barr, Max-Planck-Institute of Biochemistry, Dept. of Cell Biology, Am Klopferspitz 18a, 82152 Martinsried, Germany. Tel.: 49-89-8578-3135. Fax: 49-89-8578-3102. email: barr{at}biochem.mpg.de

We have investigated the function of mitotic kinesin-like protein (MKlp) 2, a kinesin localized to the central spindle, and demonstrate that its depletion results in a failure of cleavage furrow ingression and cytokinesis, and disrupts localization of polo-like kinase 1 (Plk1). MKlp2 is a target for Plk1, and phosphorylated MKlp2 binds to the polo box domain of Plk1. Plk1 also binds directly to microtubules and targets to the central spindle via its polo box domain, and this interaction controls the activity of Plk1 toward MKlp2. An antibody to the neck region of MKlp2 that prevents phosphorylation of MKlp2 by Plk1 causes a cytokinesis defect when introduced into cells. We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis.

Key Words: polo kinase; Plk1; MKlp2; central spindle; rabkinesin

The online version of this article includes supplemental material.

J. Sutcliffe's present address is Adolph Butenandt Institut, Lehrstuhl für Molekularbiologie, Schillerstr. 44, 80336 Munich, Germany.

Abbreviations used in this paper: MKlp, mitotic kinesin-like protein; Plk1, polo-like kinase 1; siRNA, small interfering RNA.


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