JCB logo
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 15 September 2003. doi:10.1083/jcb.200302175
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakata, T.
Right arrow Articles by Hirokawa, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakata, T.
Right arrow Articles by Hirokawa, N.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2003/9/1045 $5.00
The Journal of Cell Biology, Volume 162, Number 6, 1045-1055

Article

 Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head

Takao Nakata and Nobutaka Hirokawa

Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, Japan 113-0033

Address correspondence to Nobutaka Hirokawa, Dept. of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1, Hongo, Tokyo, Japan 113-0033. Tel.: 81-3-5841-3326. Fax: 81-3-5802-8646. email: hirokawa{at}m.u-tokyo.ac.jp

Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found that KIF5 has a preference to the microtubules in the initial segment of axon. Low dose paclitaxel treatment caused missorting of KIF5, as well as axonal membrane proteins to the tips of dendrites. Microtubules in the initial segment of axons showed a remarkably high affinity to EB1–YFP, which was known to bind the tips of growing microtubules. These findings revealed unique features of the microtubule cytoskeletons in the initial segment, and suggested that they provide directional information for polarized axonal transport.

Key Words: critical angle fluorescent microscopy; paclitaxel; polarized sorting; EB1; neurons

Abbreviations used in this paper: CAFM, critical angle fluorescent microscopy; CLSM, confocal laser scan microscopy; IS, initial segment of axon; MAP, MT-associated protein; MT, microtubule; TIR, total internal reflection; VSV-G, vesicular stomatitus virus G-protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Finding the axon
Nicole LeBrasseur
J. Cell Biol. 2003 162: 959. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents